Gene Transfer of Heme Oxygenase-1 Using an Adeno-Associated Virus Serotype 6 Vector Prolongs Cardiac Allograft Survival

Figure 1

Transduction efficiency in vitro. (a) MPK cells transduced at various concentrations by rAAV6/ARAP4, a control expression vector for the reporter gene alkaline phosphatase (AP), were stained with a histochemical stain for AP 72 hours after transduction. (b) Western blot analysis of HO-1 expression by MPK cells transduced with 2.6×10⁵ VG/cell of rAAV6/HO-1 for 48 hrs (NTC, nontransduced cells). (c) Enzymatic activity of HO-1 in MPK cells transduced with 2.6×10⁵ vg/cell of rAAV6/HO-1. Values are mean ± SEM. 𝑁=3 plates NTC cells, 𝑁=4 plates rAAV6/HO-1-transduced cells. Each plate was run in triplicate (*𝑃<0.003 as compared to nontransduced control cells, NTC).

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