Gene Transfer of Heme Oxygenase-1 Using an Adeno-Associated Virus Serotype 6 Vector Prolongs Cardiac Allograft Survival

Figure 2

Gene transfer in vivo. (a) Alkaline phosphatase staining of Balb/cJ hearts after intravenous injection of 2×10¹¹ viral genome (VG) particles of rAAV6/ARAP4 as compared to untreated controls. (b) Western blot of hearts from Balb/cJ mice treated with 2×10¹² vg of rAAV6/HO-1 as compared to nontransduced controls (NTC). (c) HO-1 functional activity in hearts from Balb/cJ mice treated with 2×10¹² vg of rAAV6/HO-1 as compared to nontransduced controls (NTC). NTC, 𝑁=3, rAAV6/HO-1, 𝑁=4. Each sample was run in triplicate (NTC) or quadruplicate (rAAV6/HO-1, *𝑃<0.05 as compared to nontransduced control cells, NTC).

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