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| Bank number | Processing media | Antibiotic regimen | Incubation protocol | Cryopreservation method | Storage condition and duration |
|
| E1 | Until 2004: M199
2004 onwards: RPMI-1640 | Vancomycin: 500 ug/mL
Gentamycin: 50 ug/mL
Piperacillin: 500 ug/mL
Nystatin: 2500 U/mL | Room temperature, 24 hours, in the dark | Controlled rate freezing | Liquid nitrogen vapour phase: 5 years |
|
| E2 | RPMI-1640 with glutamine
Addition of 20% human albumin and DMSO for cryopreservation and storage | Cefoxitin: 240 ug/mL
Lincomycin: 120 ug/mL
Colimycin: 100 ug/mL
Vancomycin: 50 ug/mL | 4°C, 24 hours | Controlled rate freezing from +10°C to −110°C | Liquid nitrogen vapour phase: 5 years |
|
| E3 | Transport solution: TRIS buffered isotonic saline
Antibiotics media: M199
Cryopreservation media: HBSS with 25 mM HEPES + 20% DMSO | Gentamicin: 4000 ug/mL
Imipenem: 200 ug/mL
Nystatin: 2500 U/mL
Polymyxin B: 200 ug/mL
Vancomycin: 50 ug/mL | 2–8°C, 18–24 hours | Controlled rate freezing (at −1°C/min) | −135°C: 10 years |
|
| E4 | Cryopreservation media: HBSS with 25 mM HEPES + 10% DMSO | Vancomycin: 50 ug/mL
Gentamicin: 4000 ug/mL
Ciprofloxacin: 200 ug/mL
Amphotericin B: 50 ug/mL | Room temperature (21°C), 24 hours | Controlled rate freezing | Liquid nitrogen vapour phase: 10 years |
|
| E5 | Antibiotics media: RPMI-1640
Cryopreservation media: RPMI-1640 + 10% DMSO | Metronidazol: 50 ug/mL
Vancomycin: 50 ug/mL
Amikacin: 50 ug/mL
Amphotericin B: 5 ug/mL | 4°C, 24 hours | Controlled rate freezing | Liquid nitrogen vapour phase: 15 years |
|
| E6 | Cryopreservation media: 70% TC-199 + 10% DMSO + 20% human albumin | Vancomycin: 50 ug/mL
Tobramycin: 50 ug/mL Cotrimoxazole: 50 ug/mL | 4°C, 6–24 hours | Controlled rate freezing (at −1°C/min) | Liquid nitrogen vapour phase: 10 years |
|
| E7 | Cryopreservation media: M199 in the past, use sodium chloride solution + 10% DMSO at present | Fluconazole: 200 mg
Cefotaxime: 1 g | 4°C, 24 hours | Liquid nitrogen vapour phase | Liquid nitrogen vapour phase: 2 years |
|
| E8 | Antibiotic media: 0.9% sodium chloride
Cryopreservation media: RPMI-1640 + 10% cryoprotectant | Metronidazole: 20 ug/mL
Gentamicin: 20 ug/mL
Flucloxacillin: 20 ug/mL | 4°C, at a minimum of 12 hours | Controlled rate freezing (down to −180°C) | Liquid nitrogen vapour phase: 5 years |
|
| E9 | M199 with Hanks' salts, L-glutamine, 25 mM HEPES | Lincomycin,
polymyxin B sulphate,
vancomycin | 4°C, 48 hours | No response provided | Liquid nitrogen vapour phase: 5 years |
|
| E10 | M199
Antibiotics media: 200 mL of Earles salt in M199 | Amphotericin B: 250 ug/mL
Fungoral: 100 ug/mL
Colistin: 200 ug/mL
Vancocin: 500 ug/mL
Garamycin: 530 ug/mL | 4°C–8°C, 24 hours | Controlled rate freezing | Liquid nitrogen vapour phase: 10 years |
|
| E11 | Transport solution: Ringer’s lactate or cardioplegia solution
Antibiotic media: M199
Cryopreservation media: M199 + Antibiotics + 12.5% DMSO | Cefuroxime: 250 ug/mL
Gentamicin: 80 ug/mL
Ciprofloxacin: 200 ug/mL
Vancomycin: 500 ug/mL
Colistin: 1000 IU/mL
Amphotericin B: 20 ug/mL | 37°C, 18–24 hours | Controlled rate freezing (from +4°C to −60°C at 1°C per min; then transfer to ultralow temperature freezer at −140°C) | Ultralow temperature (−140°C): 5 years or −80°C: for up to 6 months |
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