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| Bank number | Processing media | Antibiotic regimen | Incubation protocol | Cryopreservation method | Storage condition and duration |
|
| A1 | Heart collection, transport, dissection solution:
Hartmann solution
Antibiotic media: M199
Cryopreservation media:
M199 + 10% DMSO | Amoxicillin: 20 ug/mL
Gentamicin: 20 ug/mL | 37°C, 6–8 hours or 4°C, 18–24 hours | Controlled rate freezing | Liquid nitrogen vapour phase: 5 years |
|
| A2 | Heart collection, transport, dissection solution:
saline or M199
Antibiotic media: M199
Cryopreservation media:
M199 + 10% DMSO | Penicillin: 50 IU/mL
Streptomycin: 50 ug/mL | 35°C, 6–8 hours | Controlled rate freezing (back up: manual controlled rate freezing) | Liquid nitrogen vapour phase: 5 years |
|
| A3 | Heart collection, transport, dissection solution:
DCD/multiorgan donors/live donors—saline
Cadaveric—saline or M199 with antibiotics
Cryopreservation media:
M199 + 10% DMSO | Penicillin: 50 IU/mL
Streptomycin: 50 ug/mL | 37°C, 6–12 hours | Controlled rate freezing | Liquid nitrogen vapour phase: 10 years |
|
| A4 | Heart collection, transport, dissection solution:
Hartmann solution
Antibiotic media: M199
Cryopreservation media:
M199 + 10% DMSO | Benzylpenicillin: 30 ug/mL
Gentamicin: 18 ug/mL | 37°C, 6 hours | Controlled rate freezing | Liquid nitrogen vapour phase: 5 years |
|
| A5 | Heart collection, transport, dissection, rinse solution:
HBSS (preferred) or saline
Antibiotic media: HBSS
Cryopreservation media:
Until Jan 2010—M199 + 10% DMSO
After Jan 2010—HBSS + 10% DMSO | Cefoxitin: 240 ug/mL
Lincomycin: 120 ug/mL
Polymyxin B: 100 ug/mL
Vancomycin: 50 ug/mL
Amphotericin B: 25 ug/mL | First soak: 4°C, 24 hours;
2nd soak: 4°C, 24 hours;
transfer to HBSS at 4°C until frozen | Freezing in liquid nitrogen vapour—no controlled rate equipment used | Liquid nitrogen vapour phase: 5 years |
|
| A6 | Transport, dissection, incubation solution: M199
Cryopreservation media:
M199 + 100% DMSO diluted to 10% | Vancomycin: 50 ug/mL
Amikacin: 100 ug/mL | 4°C, 24 hours | Controlled rate freezing | Liquid nitrogen vapour phase: 5 years |
|
| A7 | M199
11% DMSO | Mefoxin: 50 ug/mL
Piperacillin: 50 ug/mL
Amikacin: 25 ug/mL
Amphotericin B: 2.5 ug/mL | 4°C, 12–18 hours | Controlled rate freezing | Liquid nitrogen vapour phase: 5–8 years |
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