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Journal of Veterinary Medicine
Volume 2015, Article ID 921868, 7 pages
http://dx.doi.org/10.1155/2015/921868
Research Article

Detection of Mycobacterium bovis in Organs of Slaughtered Cattle by DNA-Based Polymerase Chain Reaction and Ziehl-Neelsen Techniques in Bauchi State, Nigeria

1Department of Veterinary Public Health and Preventive Medicine, Ahmadu Bello University, PMB 1013, Zaria 2222, Kaduna State, Nigeria
2Area Veterinary Clinic (Kofar Ran), Ministry of Animal Resources and Nomadic Resettlement, Bauchi State, Nigeria
3TB-Laboratory, Department of Medicine, Faculty of Veterinary Medicine, Ahmadu Bello University, PMB 1013, Zaria 2222, Kaduna State, Nigeria
4Department of Medicine Faculty of Veterinary Medicine, Ahmadu Bello University, PMB 1013, Zaria 2222, Kaduna State, Nigeria
5University of Maiduguri Teaching Hospital, PMB 1069, Maiduguri, Borno State, Nigeria

Received 16 August 2014; Accepted 11 November 2014

Academic Editor: Carlos González-Rey

Copyright © 2015 A. S. Sa’idu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Bovine tuberculosis is a chronic, infectious, and contagious zoonotic disease of domestic animals, wild animals, and humans. It also poses a public health threat and economic losses. This study was aimed at determining the prevalence of bovine tuberculosis in slaughtered cattle, based on PM meat inspection, Ziehl-Neelsen staining, and PCR techniques in Bauchi State, Nigeria. A Prospective study was conducted on 800 cattle slaughtered in the three Zonal abattoirs of Bauchi State, Nigeria. One hundred and twenty (15%) tissues from different organs had suspected bTB lesions at PM. Out of the samples examined 35 (29.2%) were AFB positive by ZN and 10 (8.3%) were confirmed positive for M. bovis by PCR, with an overall prevalence of 29.16% and 8.33%, respectively. Female had a higher prevalence rate than male cattle at 16.66% and 12.5 % by ZN and 5.00% and 3.33% by PCR, respectively ( , ). However, there was a statistically significant association ( , ) between detection of bTB and the age of cattle. ZN revealed that cattle aged 6 years and above had the highest number of positive bTB cases 67.9%, while cattle aged 3–5 years had the lowest 14.81%. PCR technique revealed that the cattle aged 6 and above years also had the highest percentage positive M. bovis cases of 22.84%, whereas cattle aged 3–5 years had the lowest and the overall prevalence rate of 8.33%. The study found a high infection rate of bTB among cattle and majority of the lesions 54.2% were from lungs. The prevalence of bTB was higher in Bauchi metropolitan abattoir which supplies larger population of the state with beef.