DNA Barcoding and Phylogeny of Acari Species Based on ITS and COI Markers

Figure 3

Internal transcribed spacer (ITS) alignment fingerprint. In this case, the structure of the sequenced fragment has been included at the top of the figure. The first row (nucleotides) shows the nucleotide composition for the consensus sequence obtained from the alignment. The second row depicts the heterogeneity of the sequences in each nucleotide position. The third row (identity) depicts constant, variable, and gaps present in the alignment. The fourth row (heterozygosity) depicts the frequency of nucleotides in each position. And finally, the fifth row (diversity) represents how variable is each site within the sequence by using the Nei and Li [80] nucleotide diversity measure. As can be shown, the region from 2600 to 3054 is black (for nucleotides, heterogeneity, and identity) or red (for heterozygosity and diversity) colored, meaning that this region is only present in less than 1% of sequences. Noticeable is the central region of the alignment, which shows the most informative sites.