A New Interactive Web-Based Polytomous Key for Species Identification of Pin Nematodes of the Genus Paratylenchus Micoletzky, 1922 (Nematoda: Paratylenchinae) with the Use of Ribosomal and Mitochondrial Genes

Table 1

Morphometric and morphological characters used to distinguish Paratylenchus spp. in the web-assisted polytomous key. Some characters are ranked in the program (shown in the table), and the rest are ranked to 3.

(A) Female stylet length (rank 5); A1: <23.5 μm; A2: 23.5-40.0 μm; A3: 40.1-69.5 μm; A4: >69.5 μm; (B) Excretory pore level; B1: at median bulb level or anterior; B2: at isthmus level; B3: at basal bulb level or posterior; (C) Number of lines in the lateral field (rank 4); C1: 2 lines; C2: 3 lines; C3: 4 lines; C4: indistinct lines; (D) Advulval flap (rank 4); D1: present; D2: absent; (E) Lip region shape in lateral view; E1: conoid; E2: rounded; E3: disc-shape; E4: truncate, concave, anteriorly flattened; E5: cap-like structure; (F) Submedian lobes in lateral view; F1: without submedian lobes; F2: with small submedian lobes; F3: with prominent submedian lobes; (G) Vulva-anus distance; G1: ≤32.75 μm; G2: 32.76-47.50 μm; G3: >47.50 μm; (H) Spermatheca shape; H1: rounded to spherical; H2: elongated, oval; H3: not developed, inconspicuous

(I); I1: ≤12.20%; I2: 12.21-21.8%; I3: >21.8%; (J) Male; J1: unknown; J2: present; (K) Male tail shape; K1: conoid; K2: rounded, cylindrical; K3: filiform terminus; K4: unknown; (L) Male stylet; L1: unknown; L2: without stylet; L3: with stylet; (M) c’ ratio; M1: ≤2.25; M2: 2.26-2.85; M3: 2.86-3.35; M4: 3.36-3.95; M5: >3.95; (N) b ratio; N1: ≤2.85; N2: 2.86-3.65; N3: 3.66-4.25; N4: >4.25; (O) Body length (L); O1: ≤253 μm; O2: 253-303 μm; O3: 304-356 μm; O4: 357-426 μm; O5: >426 μm; (P) Spicules’ length; P1: ≤14.25 μm; P2: 14.26-16.75 μm; P3: 16.76-19.75 μm; P4: 19.76-23.0 μm; P5: >23.0 μm

(Q) Female tail terminus shape; Q1: acute or pointed; Q2: subacute to finely rounded; Q3: bluntly rounded; Q4: digitate, lobed, or indented; Q5: serrated; (R) V ratio (%); R1: ≤76.0%; R2: 76.1-81.0%; R3: >81.0%; (S) Female tail length; S1: ≤26.4 μm; S2: >26.4 μm; (T) J4 stylet; T1: absent; T2: present; T3: unknown; (U) c ratio; U1: ≤13.25; U2: 13.26-17.25; U3: >17.25; (V) Female tail shape; V1: conoid; V2: cylindrical; V3: subcylindrical, elongate; (W) Cuticle annulation; W1: normal annulation, ca. 1 μm; W2: cuticle with punctations; W3: cuticle annuli crenate; (X) a ratio; X1: ≤21.75; X2: >21.7