A New Interactive Web-Based Polytomous Key for Species Identification of Pin Nematodes of the Genus Paratylenchus Micoletzky, 1922 (Nematoda: Paratylenchinae) with the Use of Ribosomal and Mitochondrial Genes
Table 2
Species complexes included in the polytomous key with molecular markers needed for putative species separation. Many of these putative species are not formally described as a new species. Species separation data were obtained from Singh et al. [3] and Clavero-Camacho et al. [4, 5].
Species
D2-D3 regions
COI
n. speciesa
% similarity
Seq. GenBankb
Suitabilityc
n. speciesa
% similarity
Seq. GenBankb
Suitabilityc
P. aquaticus
2
73.2-98.5
4 (4)
Yes
2
81.1
2 (2)
Yes
P. enigmaticus
2
99.8-100
17 (2)
No
3
97.1-100
7 (4)
Yes
P. goodeyi
1
98.3-100
32 (9)
No
7
94.3-100
19 (15)
Yes
P. hamatus/P. sp. 2/P. sp. CaD/P. tenuicaudatus
4
94.4-100
49 (15)
Yes
4
93.7-100
17 (4)
Yes
P. sheri
1
99.6-100
6 (3)
No
2
94.3-100
9 (3)
Yes
P. straeleni/P. parastraeleni
6
93.0-100
14 (9)
No
10
91.1-100
29 (14)
Yes
P. veruculatus
2
97.4-100
17 (7)
No
3
93.7-100
13 (6)
Yes
aBased on species separation methods [3] or similarity cut-off (approx. 98% for D2-D3 regions and 97.5% for the COI fragment). bNumber of different haplotypes in brackets. cSuitability to separate species inside the species-complex taking the number of species with COI marker for the D2D3 regions. This marker cannot separate P. sheri from P. israelensis and P. neoamblycephalus.
