Reevaluation of the Systematic Status of Branchinotogluma (Annelida, Polynoidae), with the Establishment of Two New Species

Table 1

Sampling information of the species and corresponding accession numbers used in this study. New sequences are set in boldface.

Species	Voucher	Gener	Vent and seep location	COI	16S	18S	28S	References
Bathykurila guaymasensis	—	—	Whale fall off San Diego & Santa Cruz Basin, California	DQ074766	MN428326	DQ074765	—	Glover et al. [20]; Hatch et al. [16]
Branchinotogluma bipapillata	RSIO35274	—	Southwest Indian Ocean Ridge	MH712481	MH717076	MH717080	—	Zhou et al. [4]
Branchinotogluma sandersi	SMNH118960	—	Juan de Fuca Ridge, eastern Pacific	JN852923	JN852889	JN852821	JN852851	Norlinder et al. [14]
Branchinotogluma elytropapillata	NSMT-pol P-668	Female	Sakai field, Okinawa Trough	MG799387	MG799377	MG799378	MG799380	Zhang et al. [8]
Branchinotogluma hessleri	SIO:BIC:A6316	—	Southern Gulf of California	KY684713	MH127414	MH124626	MH124616	Goffredi et al. [15]; Hatch et al. [16]
Branchinotogluma japonicus	—	—	Sakai vent field, Okinawa Trough	KY753824	KY753824	KY753841	KY753841	Zhang et al. [8]
	NSMT-Pol 113241	Male	Sakai field, Okinawa Trough, western Pacific	MG799390	—	—	—	Zhang et al. [8]
	NSMT-Pol 113242	Male	Sakai field, Okinawa Trough, western Pacific	MG799391	—	—	—	Zhang et al. [8]
	CBM-ZW 1114	Female	Aki site, Okinawa Trough, western Pacific	MG799392	—	—	—	Zhang et al. [8]
Branchinotogluma marianus	SIO:BIC:A13116	—	—	MW646931	MW654556	MW654525	—	Unpublished
Branchinotogluma nanhaiensis sp. nov.	MBM286800-sps4	—	Seep, South China Sea	OL702996	OL699914	OL699911	OL699917	This study
	MBM286796-sps3	Male	Seep, South China Sea	OL702999	—	—	—	This study
	MBM286797-sps3	Male	Seep, South China Sea	OL703002	—	—	—	This study
	MBM286797-sps4	Male	Seep, South China Sea	OL703000	—	—	—	This study
	MBM286797-sps5	Male	Seep, South China Sea	OL703001	—	—	—	This study
	MBM286800-sps1	Female	Seep, South China Sea	OL702997	—	—	—	This study
	MBM286800-sps5	Female	Seep, South China Sea	OL703003	—	—	—	This study
	MBM286800-sps6	Female	Seep, South China Sea	OL702998	—	—	—	This study
Branchinotogluma nikkoensis	—	—	Nikko seamount	ON255504	ON244617	ON244619	ON244615	Jimi et al. [6]
Branchinotogluma ovata	MBM286062-sps2	Male	Manus back-arc basin	MK357896	MK211416	MK211411	MK211413	Wu et al. [10]
Branchinotogluma pettiboneae	MBM286047	Female	Vent, manus back-arc basin, tropical western Pacific	OL703004	OL699915	OL699912	OL699918	This study
	MBM286050-sps1	Male	Vent, manus back-arc basin, tropical western Pacific	MK357901	—	—	—	Wu et al. [10]
	MBM286051-sps2	Female	Vent, manus back-arc basin, tropical western Pacific	MK357902	—	—	—	Wu et al. [10]
	MBM286052	Male	Vent, manus back-arc basin, tropical western Pacific	MK357903	—	—	—	Wu et al. [10]
	MBM286049-sps1	Female	Vent, manus back-arc basin, tropical western Pacific	MK357904	—	—	—	Wu et al. [10]
Branchinotogluma robusta sp. nov.	MBM286805-sps2	Female	Seep, South China Sea	OL703005	OL699916	OL699913	OL699919	This study
	MBM286803-sps1	Female	Seep, South China Sea	OL703007	—	—	—	This study
	MBM286803-sps2	Female	Seep, South China Sea	OL703008	—	—	—	This study
	MBM286804-sps1	Female	Seep, South China Sea	OL703009	—	—	—	This study
	MBM286804-sps2	Female	Seep, South China Sea	OL703010	—	—	—	This study
	MBM286805-a	Female	Seep, South China Sea	OL703012	—	—	—	This study
	MBM286805-b	Male	Seep, South China Sea	OL703013	—	—	—	This study
	MBM286805-c	Male	Seep, South China Sea	OL703014	—	—	—	This study
	MBM286805-d	Female	Seep, South China Sea	OL703015	—	—	—	This study
	MBM286805-sps3	Female	Seep, South China Sea	OL703011	—	—	—	This study
	MBM286805-sps5	Male	Seep, South China Sea	OL703006	—	—	—	This study
	MBM286806	?	Seep, South China Sea	OL703016	—	—	—	This study
Branchinotogluma sagamiensis	—	—	Off Hatsushima, Sagami Bay	ON255503	ON244616	ON244618	ON244614	Jimi et al. [6]
Branchinotogluma sandersi	SIO:BIC:A6321	—	Southern Gulf of California	KY684716	MH127416.1	MH124627.1	MH124617.1	Goffredi et al. [15]; Hatch et al. [16]
Branchinotogluma segonzaci	—	—	Fiji, Manus back-arc basin	MK357906	MK694858	MK211412	—	Wu et al. [10]
Branchinotogluma trifurcus	—	—	Manus back-arc basin	MK357905	MK211415	MW654529	—	Wu et al. [10]
Branchinotogluma tunnicliffeae	SIO:BIC:A7717	—	—	MW646935	MW654560	MW654530	MW654552	Unpublished
Branchiplicatus cupreus	SIO:BIC:A6160	—	Southern Gulf of California	KY684706	MH127418	MH124628	—	Goffredi et al. [15]; Hatch et al. [16]
Branchipolynoe eliseae	SIO–BIC A6548	—	Costa Rica	MH369878	MH396826	—	—	Lindgren et al. [21]
Branchipolynoe halliseyae	SIO–BIC A6532	—	Costa Rica	MH369858	MH396795	—	—	Lindgren et al. [21]
Branchipolynoe kajsae	SIO–BIC A2161	—	Costa Rica	MH369859	MH396800	—	—	Lindgren et al. [21]
Branchipolynoe longqiensis	—	—	Longqi field, Southwest Indian Ocean Ridge	KY753826	KY753826	KY753847	KY753847	Zhang et al. [17]
Branchipolynoe meridae	SIO–BIC A2131	—	Costa Rica	MH369884	MH396829	—	—	Lindgren et al. [21]
Branchipolynoe pettiboneae	—	—	South China Sea, western Pacific	KY753825	KY753825	KY753840	KY753840	Zhang et al. [17]
Branchipolynoe seepensis	SIO–BIC A6553	—	Gulf of Mexico, USA	MH369885	MH596848	—	—	Lindgren et al. [21]
Branchipolynoe symmytilida	—	—	East Pacific Rise	AY646021	AF315055	—	—	Halanych et al. [22]; Hurtado et al. [23]
Branchipolynoe tjiasmantoi	SIO–BIC A8511	—	Lau back-arc basin	MH369947	MH396830	—	—	Lindgren et al. [21]
Halosydnella australis	KW402	—	Brazil	KY823495	KY823480	KY823449	KY823463	Gonzalez et al. [24]
Harmothoe impar	SMNH118968	—	Sweden	JN852930	JN852897	JN852829	JN852859	Norlinder et al. [14]
Lepidonotopodium fimbriatum	SIO–BIC A6153	—	Gulf of California, Mexico	KY684717	MN428327	MN428333	MN428320	Goffredi et al. [15]; Hatch et al. [16]
Lepidonotopodium okinawae	—	—	Sakai vent field, Okinawa Trough	KY753828	KY753828	KY753842	KY753842	Zhang et al. [17]
Lepidonotopodium sp. n.	SIO–BIC A6317	—	Gulf of California, Mexico	KY684715	MN428328	MN428334	MN428321	Goffredi et al. [15]; Hatch et al. [16]
Lepidonotopodium williamsae	SIO–BIC A6318	—	Gulf of California, Mexico	KY684714	MN428329	MN428335	MN428322	Goffredi et al. [15]; Hatch et al. [16]
Lepidonotus clava	SMNH118974		England	JN852934	JN852902	JN852833	JN852864	Norlinder et al. [14]
Levensteiniella iris	—	—	East Scotia Ridge	KY753827	KY753827	KY753848	KY753848	Zhang et al. [17]
Levensteiniella manusensis	MBM286000-sps5	Juvenile	Vent, manus back-arc basin, tropical western Pacific	OP114410	OP144063	OP144059	OP144061	This study
Levensteiniella undomarginata	CBM-ZW 1118	Male	Sakai field, Okinawa Trough	MG799385	MG799376	MG799379	MG799381	Zhang et al. [8]
Peinaleopolynoe elvisi	SIO–BIC A8488	—	California, USA	MH115413	MH127422	MH124629	MH124618	Hatch et al. [16]
Peinaleopolynoe goffrediae	SIO–BIC A5485	—	California, USA	MN431782	MN428330	MN428336	MN428323	Hatch et al. [16]
Peinaleopolynoe mineoi	SIO–BIC A9919	—	Costa Rica	MN431773	MN428331	MN428337	MN428324	Hatch et al. [16]
Peinaleopolynoe orphanae	SIO–BIC A6151	—	Gulf of California, Mexico	KY684727	MH127423	MH124630	MH124619	Goffredi et al. [15]; Hatch et al. [16]
Peinaleopolynoe santacatalina	SIO–BIC A8487	—	California, USA	MH115411	MH127413	MH124621	MH124612	Hatch et al. [16]
Peinaleopolynoe sillardi	MNHN–IA–2010–399	—	South Atlantic Ocean	MH115414	MH127421	—	—	Hatch et al. [16]
Thermopolynoe branchiata	MBM286003	Male	Vent, Manus back-arc basin, tropical western Pacific	OP114411	OP144064	OP144060	OP144062	This study