Table of Contents
Leukemia Research and Treatment
Volume 2012 (2012), Article ID 482905, 17 pages
http://dx.doi.org/10.1155/2012/482905
Research Article

PKCδ Regulates Translation Initiation through PKR and eIF2α in Response to Retinoic Acid in Acute Myeloid Leukemia Cells

1Department of Experimental Therapeutics, Unit 422, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA
2Department of Leukemia, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA
3Department of Bioinformatics and Computational Biology, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA

Received 14 February 2012; Revised 8 May 2012; Accepted 10 May 2012

Academic Editor: George P. Studzinski

Copyright © 2012 Bulent Ozpolat et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure 1: Morphology and cellular structures of NB4 cells. Cells were analyzed by May-GrΓΌnwald-Giemsa staining (upper panels) and transmission electron microscopy (lower panels), respectively, before and after 72 h of ATRA (1 μM) treatment.

Supplementary Figure 2: ATO at high concentrations induces apoptosis in APL cells. NB4 cells were treated with ATO (2 μM) for 24 h and collected for Western blot analysis. ATO induced apoptosis in NB4 cells as indicated by cleavage (activation) of caspases 9, 3 and PARP cleavage.

Supplementary Figure 3: Knockdown of PERK by siRNA has no effect on phosphorylation of eIF2Ξ± in NB4 cells. Cells were transfected with PERK or control siRNA for 48 h. 24 h after ATRA treatment cells were collected, lysed and analyzed by Western blot.

  1. Supplementary Figures