Cryptolepine-Induced Cell Death of Leishmania donovani Promastigotes Is Augmented by Inhibition of Autophagy
Figure 8
(a) Real-time PCR analysis for the expression level of ATG 8 and Sir2 genes from L. donovani promastigotes. The expression of ATG 8 and Sir2 were estimated relative to GAPDH in the treated samples compared with the untreated control. The fold expression was calculated as described in Section 2. The mean fold expression values are given in Table 2. Data are represented as Mean ± SEM (). (b) Fragmentation of genomic DNA in the presence and in the absence of 3-MA and CLP for different time periods. Genomic DNAs were isolated from L. donovani promastigotes after treatment with 0.2% DMSO alone for 2 h (lane 1), 10 mM 3-MA for 2 h (lane 2), 20 μM CLP for 2 h (lane 3), and 20 μM CLP for 2 h after pretreatment with 10 mM 3-MA (lane 4). Lanes 5–8, the same as lanes 1–4, respectively, but for 6 h.
