Primer Based Approach for PCR Amplification of High GC Content Gene: Mycobacterium Gene as a Model
Figure 2
Amplification pattern of 16S rDNA, Rv0519, and Rv0774c genes from M. tuberculosis (Figures 2(a) and 2(b)), ML0314c and 16S rDNA from M. leprae (Figures 2(c) and 2(d)). (a) Amplification of 16S rDNA, Rv0519, Rv0774c genes with standard primers, (b) amplification of Rv0519 with modified primers, (c) amplification of 16S rDNA and ML0314c genes with standard primers, and (d) amplification of ML0314c with modified primers.
