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Mediators of Inflammation
Volume 1 (1992), Issue 4, Pages 263-266

Turnour necrosis factor stimulates endothelin-1 gene expression in cultured bovine endothelial cells

1Mario Negri Institute for Pharmacological Research, Via Gavazzeni 11, Ospedali Riuniti di Bergamo, Bergamo 24100, Italy
2Division of Nephrology, Ospedali Riuniti di Bergamo, Bergamo 24100, Italy

Copyright © 1992 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


We have studied the effect of human recombinant tumour necrosis factor-α (TNF-α) on gene expression and production of endothelin-1 in cultured bovine aortic endothelial cells. TNF-α (10 and 100 ng ml−1) increased in a time dependent manner the preproendothelin-1 mRNA levels in respect to unstimulated endothelial cells. TNF-α induced endothelin-1 gene expression was associated with a parallel increase in the release of the corresponding peptide in the culture medium. These findings suggest that the enhanced synthesis and release of endothelin-1 occurring in conditions of increased generation of TNF, may act as a modulatory factor that counteracts the hypotensive effect and the excessive platelet aggregation and adhesion induced by TNF.