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Mediators of Inflammation
Volume 2, Issue 3, Pages 229-233
http://dx.doi.org/10.1155/S0962935193000316

Antibodies directed to antigens secreted by murine epithelioid macrophages modulate BCG-induced granulomata

1Department of Immunology, Institute of Biomedical Sciences, University of São Paulo, SP, Brazil
2Ludwig Institute for Cancer Research, São Paulo branch, São Paulo, SP, Brazil
3Department of Pathology, Adolpho Lutz Institute, SP, Brazil
4Department of Biochemistry and Molecular Biology, Oswaldo Cruz Institute, Rio de Janeiro, RJ, Brazil

Received 24 March 1993; Accepted 1 April 1993

Copyright © 1993 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The authors have previously shown that epithelioid cells isolated from mice secrete a factor, called macrophage deactivating factor (MDF), that promptly deactivates superoxide release by activated macrophages and neutrophils. In this paper some biological properties of a polyclonal rat antiserum directed to MDF and other substances secreted by these cells are described. The immunoglobulin fraction of this antiserum reacted, by immunocytochemical methods, with epitopes in the cell membrane of macrophages adherent to coverslips subcutaneously implanted for 14 days; but not for 5 days. It also reacted with antigens within and outside cells in BCG-induced granulomas. This antiserum blocked completely the macrophage deactivating activity of epithelioid cell culture supernatants. Anti-IL-10 monoclonal antibody, did not block MDF activity. The administration of the immunoglobulin fraction from immunized rats to C5 deficient mice bearing BCG-induced granulomatas in the footpad, significantly reduced the size of the lesions. A marked necrosis of inflammatory cells and mononuclear cells phagocyting debris of necrotic cells were observed in these lesions.