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Mediators of Inflammation
Volume 3 (1994), Issue 1, Pages 27-31
Research paper

Interferon-γ Stimulates Monocyte Chemotactic Protein-1 Expression by Monocytes

1Department of Pathology, University of Michigan, Medical School, Ann Arbor, Michigan, USA
2Department of Internal Medicine, University of Michigan, Medical School, Ann Arbor, Michigan, USA
3Department of Medicine, Oregon Health Sciences University, 3181 S.W. Sam Jackson Park Road, Portland, OR 97201-3098, USA

Received 28 September 1993; Accepted 16 November 1993

Copyright © 1994 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Monocyte chemotactic protein (MCP-1) is a specific monocyte chemoattractant and activating factor produced by both immune cells (mononuclear phagocytes and lymphocytes) and non-immune cells (parenchymal and stromal cells). In order to define the conditions under which human monocytes express MCP-1, monocytes were exposed to IFN-γ, IL- lβ, TNF-α, IL-4 or PHA under serum free conditions. There was no significant MCP-1 production by monocytes following exposure to IL-lβ, TNF-α or IL-4. In contrast, stimulation with IFN-γ resulted in a dose dependent increase in MCP-1 protein and mRNA expression. Simultaneous stimulation with IFN-γ and IL-1β or TNF-α resulted in no further increase in MCP-1 production. It is concluded that IFN-γ, primarily a product of TH1 T lymphocytes, stimulates the expression of MCP-1 by monocytes.