Abstract
The study tests the role of thromboxane in modulating microvascular
permeability in vitro. Cultured monolayers of bovine
aortic endothelial cells were challenged with the thromboxane (Tx)
mimic U46619. This led to disassembly of actin microfilaments, cell
rounding, border retraction and interendotheHal gap formation.
Pretreatment with the Tx receptor antagonist SQ 29,548 prevented the
Tx mimic-induced cytoskeletal changes. The Tx mimic also altered
endothelial cell barrier function. Increased permeability was
indicated by the increased passage of labelled albumin across
monolayers cultured on microcarriers, relative to untreated
endothelial cells (