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Mediators of Inflammation
Volume 3, Issue 3, Pages 205-209

Changes and Relationship of PAF and TNF in Rats with Myocardial Ischaemia and Reperfusion Injury

1Department of Cardiology, Chang Zheng Hospital, Shanghai, China
2Department of Pathophysiology, Second Military Medical Universit,, Shanghai, China
3Department of Pharmacology (School of Pharmacy), Second Military Medical University, Shanghai, China

Copyright © 1994 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


In this study it is reported that: (1) the levels of blood platelet-activating factor and serum tumour necrosis factor significantly increased after coronary ligation and reperfusion, compared with sham-ligated controls, in an anaesthetized rat model; (2) compared with vehicle controls, pretreatment with the PAF antagonist BN 50739 (10 mg/kg, i.v.) produced significant decreases in infarct size (from 29.6 ± 4.0% to 22.4 ± 2.1%, p<0.05 after 3 h ligation, and from 28.5 ± 9.5% to 10.5 ± 4.5%, p<0.01 after 4 h reperfusion) and the level of serum TNF (from 10.4 ± 7.7 U/ml to 3.9 ± 4.8 U/ml, p<0.05); and (3) a significan positive correlation was found between the level of blood PAF or serum TNF and infarct size. The present results indicate that PAF and TNF may be important mediators involved in myocardial ischaemia and reperfusion injury, and that PAF antagonists may exert a protective effect on ischaemic or reperfused myocardium by inhibiting the interaction of PAF and TNF.