Mediators of Inflammation

Mediators of Inflammation / 1996 / Article

Open Access

Volume 5 |Article ID 196865 |

J. M. Huebner, R. R. Eversole, W. F. Jackson, C. D. Mackenzie, S. R. Stapleton, L. J. Beuving, "Leukotriene C4 biosynthesis in isolated August rat peritoneal leukocytes", Mediators of Inflammation, vol. 5, Article ID 196865, 5 pages, 1996.

Leukotriene C4 biosynthesis in isolated August rat peritoneal leukocytes


The mixed leukocyte population obtained from the peritoneum of the August rat is a potentially important experimental model of inherent eosinophilia that has not been well characterized. In the present study, isolated cell preparations generated a concentration-dependent release of leukotriene (LT) C4 when exposed to the Ca2+ ionophore A23187, reaching maximal stimulation at 5.0 μM. This response was inhibited by the 5-lipoxygenase activating protein antagonist MK-886 (0.1 μM), nominally Ca2+ and Mg2+-free incubation media and by activation of protein kinase C via phorbol 12-myristate 13-acetate (50 nM). These findings establish a model system for investigating LTC4 profiles contingent with innate peritoneal eosinophilia and are consistent with the hypothesis that cellular LTC4 biosynthesis is phosphoregulated.

Copyright © 1996 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

More related articles

 PDF Download Citation Citation
 Order printed copiesOrder

Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.