J. M. Huebner, R. R. Eversole, W. F. Jackson, C. D. Mackenzie, S. R. Stapleton, L. J. Beuving, "Leukotriene C4 biosynthesis in isolated August rat peritoneal leukocytes", Mediators of Inflammation, vol. 5, Article ID 196865, 5 pages, 1996. https://doi.org/10.1155/S0962935196000610
Leukotriene C4 biosynthesis in isolated August rat peritoneal leukocytes
The mixed leukocyte population obtained from the peritoneum of the August rat is a potentially important experimental model of inherent eosinophilia that has not been well characterized. In the present study, isolated cell preparations generated a concentration-dependent release of leukotriene (LT) C4 when exposed to the Ca2+ ionophore A23187, reaching maximal stimulation at 5.0 μM. This response was inhibited by the 5-lipoxygenase activating protein antagonist MK-886 (0.1 μM), nominally Ca2+ and Mg2+-free incubation media and by activation of protein kinase C via phorbol 12-myristate 13-acetate (50 nM). These findings establish a model system for investigating LTC4 profiles contingent with innate peritoneal eosinophilia and are consistent with the hypothesis that cellular LTC4 biosynthesis is phosphoregulated.
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