Mediators of Inflammation

Mediators of Inflammation / 1996 / Article

Open Access

Volume 5 |Article ID 643741 |

G. S. Smith, C. Rieckenberg, W. E. Longo, D. L. Kaminski, J. E. Mazuski, Y. Deshpande, T. A. Miller, "The effect of an interleukin receptor antagonist (IL-1ra) on colonocyte eicosanoid release", Mediators of Inflammation, vol. 5, Article ID 643741, 4 pages, 1996.

The effect of an interleukin receptor antagonist (IL-1ra) on colonocyte eicosanoid release


We investigated whether an interleukin 1 receptor antagonist (IL-1ra) altered cellular release of prostanoids and leukotrienes in a transformed colonic cell line (CACO-2) in the presence of proinflammatory stimuli. Cellular inflammation was induced by treatment with lipopolysaccharide (LPS) or the cytokine, interleukin 1 beta (IL-1β). In a separate set of experiments, cells were pretreated with IL-1ra prior to exposure to LPS or IL-1β. Prostaglandin E2 and leukotriene B4 (LTB4) levels were quantified by ELISA assays. Both LPS and IL-1β exposure were noted to stimulate cellular PGE2 release, a response which was significantly inhibited by IL-1ra treatment. Either stimulant when administered alone failed to stimulate release of LTB4. When administered after IL-1ra pretreatment however, both stimuli caused a significant increase in LTB4 release. These results suggest that a cytokine receptor antagonist can selectively influence eicosanoid production in this cell line. Furthermore, this study suggests that a IL-1ra may have a future clinical role in the treatment of inflammatory disorders of the colon which are intimately linked to enhanced eicosanoid synthesis.

Copyright © 1996 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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