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Mediators of Inflammation
Volume 6, Issue 5-6, Pages 355-361
http://dx.doi.org/10.1080/09629359791497

The effect of ozone exposure on the release of eicosanoids in guinea-pig BAL fluid in relation to cellular damage and inflammation

1Leiden/Amsterdam Center for Drug Research, Department of Pharmacochemistry, Vrije Universiteit, De Boelelaan 1083, Amsterdam 1081 HV, The Netherlands
2Department of Toxic Effects, Laboratory of Health Effects Research, National Institute of Public Health and the Environment, P.O. Box 1, Bilthoven 3720 BA, The Netherlands
3Laboratory of Pathology and Immunobiology, National Institute of Public Health and the Environment, P.O. Box 1, Bilthoven 3720 BA, The Netherlands
4Department of Pharmacology, Faculty of Medicine, Erasmus University, P.O. Box 1738, Rotterdam 3000 DR, The Netherlands

Copyright © 1997 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The observed effects after ozone exposure strongly depend on ozone concentration and exposure time. We hypothesized that depending on the O3 exposure protocol, mainly either an oxidant damage or an inflammation will determine the O3 toxicity. We compared two different ozone exposure protocols: an acute exposure (3 ppm 2 h) for studying the oxidant damage and an exposure (1 ppm 12 h) where an inflammatory component is also probably involved. We measured LDH activity and protein and albumin exudation as markers for cellular damage. After the acute exposure an increase in LDH activity was measured and after exposure to 1 ppm ozone for 12 h the exudation of protein and albumin was also enhanced. The histological examinations showed a neutrophilic inflammatory response only after exposure to 1 ppm ozone for 12 h. The acute exposure protocol resulted in an increased release of PGE2, PGD2, PGF and 6-ketoPGF whereas exposure to 1 ppm ozone for 12 h led to an additional release of LTB4. No effects were measured on the release of TxB2 and LTC4/D4/E4. These changed amounts of eicosanoids will probably contribute to the ozone-induced lung function changes.