Mediators of Inflammation

Mediators of Inflammation / 1998 / Article

Open Access

Volume 7 |Article ID 412459 |

Michael Emeis, Josef Sonntag, Carsten Willam, Evelyn Strauss, Matthias M. Walka, Michael Obladen, "Acidosis activates complement system in vitro", Mediators of Inflammation, vol. 7, Article ID 412459, 4 pages, 1998.

Acidosis activates complement system in vitro


We investigated the in vitro effect of different form s of acidosis (pH 7.0) on the formation of anaphylatoxins C3a and C5a. Metabolic acidosis due to addition of hydrochloric acid (10 μ mol/ml blood) or lactic acid (5.5 μ mol/ml) to heparin blood (N=12) caused significant activation of C3a and C5a compared to control (both p=0.002). Respiratory acidosis activated C3a (p=0.007) and C5a (p=0.003) compared to normocapnic controls. Making blood samples with lactic acidosis hypocapnic resulted in a median pH of 7.37. In this respiratory compensated metabolic acidosis, C3a and C5a were not increased. These experiments show that acidosis itself and not lactate trigger for activation of complement components C3 and C5.

Copyright © 1998 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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