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Mediators of Inflammation
Volume 7 (1998), Issue 4, Pages 289-294

Ozone oxidative preconditioning: a protection against cellular damage by free radicals

1Center for Research and Biological Evaluation (Pharmacy Institute of Havana University), Havana, Cuba
2Ozone Research Center, Havana, Cuba
3National Center for Scientific Research, Havana, Cuba
4Institute of General Physiology, University of Siena, Siena, Italy

Copyright © 1998 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


There is some anecdotal evidence that oxygen-ozone therapy may be beneficial in some human diseases . However so far only a few biochemical and pharmacodynamic mechanisms have been elucidated. On the basis of preliminary data we postulated that controlled ozone administration would promote an oxidative preconditioning preventing the hepatocellular damage mediated by free radicals. Six groups of rats were classified as follows: (1) negative control, using intraperitoneal sunflower oil; (2) positive control using carbon tetrachloride (CCl4) as an oxidative challenge; (3) oxygen-ozone, pretreatment via rectal insufflation (15 sessions ) and after it, CCl4; (4) oxygen, as group 3 but using oxygen only; (5) control oxygen-ozone, as group 3, but without CCl4 ; group (6) control oxygen, as group 5, but using oxygen only. We have evaluated critical biochemical parameters such as levels of transaminase , cholinesterase , super - oxide dismutase, catalase , phospholipase A, calcium dependent ATPase, reduced glutathione , glucose 6 phosphate dehydrogenase and lipid peroxidation. Interestingly, in spite of CCl4 adminis tration, group 3 did not differ from group 1, while groups 2 and 4 showed significant differences from groups 1 and 3 and displayed hepatic damage . To our knowledge these are the first experimental results showing that repeated administration of ozone in atoxic doses is able to induce an adaptation to oxidative stress thus enabling the animals to maintain hepatocellular integrity after CCl4 poisoning.