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Mediators of Inflammation
Volume 10, Issue 6, Pages 333-337
http://dx.doi.org/10.1080/09629350120102352

Suppressive effects of anti-allergic agent suplatast tosilate (IPD-1151T) on the expression of co-stimulatory molecules on mouse splenocytes in vivo

1First Department of Internal Medicine, Research Center for Medical Sciences School of Medicine, Showa University, 1–5-8 Hatanodai, Shinagawa-ku, Tokyo 142–8666, Japan
2Department of Physiology, Research Center for Medical Sciences School of Medicine, Showa University, 1–5-8 Hatanodai, Shinagawa-ku, Tokyo 142–8666, Japan
3Division of Immunology, Research Center for Medical Sciences School of Medicine, Showa University, 1–5-8 Hatanodai, Shinagawa-ku, Tokyo 142–8666, Japan
4Department of Internal Medicine, Kikuna Memorial Hospital, Kanagawa, Japan

Copyright © 2001 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The effects of IPD-1151T on the expression of costimulatory molecules, CD40, CD80 and CD86, were investigated in vivo using mice with allergic disorders. BALB/c mice were immunized intraperitoneally with two doses of dinitrophenylated ovalbumin (DNP-OVA) at 1-week intervals. These mice then were treated intraperitoneally with 100μg/kg of IPD1151T once a day for 14 days, starting 7 days after the first immunization. On day 21, some mice were challenged intraperitoneally with DNP-OVA and the other mice were not challenged. All mice were autopsied on day 22 and assayed for immunoglobulin E, interleuken (IL)-4 and IL-5 productions following DNP-OVA immunization. The intraperitoneal treatment with IPD-1151T strongly suppressed immunoglobulin E contents in serum, which were enhanced by DNA-OVA immunization. IPD-1151T also caused a decrease in both IL-4 and IL-5 levels in splenic lymphocytes. We next examined the influence of IPD1151T on co-stimulatory molecule expression on splenic lymphocytes. IPD-1151T caused suppression of CD40 and CD86 expression; however, the treatments did not affect CD80 expression.