Aryene Goes Trezena, Zilma Lucia da Silva, Ricardo Martins Oliveira-filho, Amilcar Sabino Damazo, Anita Hilda Straus, Helio Kiyoshi Takahashi, Sonia Maria Oliani, Wothan Tavares de Lima, "Differential regulation of the release of tumor necrosis factor-α and of eicosanoids by mast cells in rat airways after antigen challenge", Mediators of Inflammation, vol. 12, Article ID 343097, 10 pages, 2003. https://doi.org/10.1080/09629350310001599684
Differential regulation of the release of tumor necrosis factor-α and of eicosanoids by mast cells in rat airways after antigen challenge
Background: Rat trachea display a differential topographical distribution of connective tissue mast cells (CTMC) and mucosal mast cells (MMC) that may imply regional differences in the release of allergic mediators such as tumor necrosis factor-α (TNF-α) and eicosanoids.Aim: To evaluate the role of CTMC and MMC for release of TNF-α and eicosanoids after allergenic challenge in distinct segments of rat trachea.Materials and methods: Proximal trachea (PT) and distal trachea (DT) from ovalbumin (OVA)-sensitized rats, treated or not with compound 48/80 (48/80) or dexamethasone, were incubated in culture medium. After OVA challenge, aliquots were collected to study release of TNF-α and eicosanoids.Results: Release of TNF-α by PT upon OVA challenge peaked at 90 min and decayed at 6 and 24 h. Release from DT peaked at 30-90 min and decayed 6 and 24 h later. When CTMC were depleted with 48/80, OVA challenge exacerbated the TNF-α release by PT at all time intervals, while DT exacerbated TNF-α levels 6 and 24 h later only. Dexamethasone reduced TNF-α production after 90 min of OVA challenge in PT and at 3 and 6h in DT. OVA challenge increased prostaglandin D2 in DT and leukotriene B4 in both segments but did not modify prostaglandin E2 and leukotriene C4 release.Conclusion: OVA challenge induces TNF-α release from MMC, which is negatively regulated by CTMC. The profile of TNF-α and eicosanoids depends on the time after OVA challenge and of the tracheal segment considered.
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