Mediators of Inflammation

Mediators of Inflammation / 2003 / Article

Open Access

Volume 12 |Article ID 575934 |

Fadia R. Homaidan, Iman Chakroun, Marwan E. El-sabban, "Regulation of nuclear factor-κB in intestinal epithelial cells in a cell model of inflammation", Mediators of Inflammation, vol. 12, Article ID 575934, 7 pages, 2003.

Regulation of nuclear factor-κB in intestinal epithelial cells in a cell model of inflammation


Background: Interleukin-1 (IL-1), an inflammatory cytokine whose levels are elevated in inflamed mucosa, causes part of its effect on intestinal epithelial cells (IEC) through inducing ceramide production.Aim: To study the role of nuclear factor-κB (NF-κB), a pro-inflammatory and anti-apoptotic factor, in IL-1- treated IEC.Methods: NF-κB activity and levels of apoptotic proteins were assessed by electrophoretic mobility shift assay and RNA-protection assay, respectively.Results: IL-1 and ceramide, which have been shown to partially mediate IL-l effects on IEC, activated NF-κB levels significantly. This activation was due to a decrease in IκB-α and IκB-β protein levels. Moreover, the ratio of mRNA levels of anti-apoptotic to pro-apoptotic proteins was significantly increased in IL-1-treated IEC. Conclusion: NF-κB may play a key role in the regulation of the expression of pro-inflammatory and/or apoptotic genes in inflammatory bowel disease, making this protein an attractive target for therapeutic intervention.

Copyright © 2003 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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