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Mediators of Inflammation
Volume 12, Issue 1, Pages 47-49

Exhaled nitric oxide in mylar balloons: influence of storage time, humidity and temperature

1Department of Pediatrics/Respiratory Medicine, Erasmus University Medical Centre, Rotterdam/ Sophia Children's Hospital, P.O. Box 2060, Rotterdam 3000 CB, The Netherlands
2Clinica Pediatrica, Universitá di Verona, Verona, Italy

Copyright © 2003 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Background: Mylar balloons are used to collect exhaled air for analysis of fractional nitric oxide concentration (FENO).

Aim: We studied the effect of storage conditions on the stability of nitric oxide (NO) in mylar balloons.

Methods: Exhaled air samples and calibration gases were stored in mylar balloons at 4, 21 and 37°C, with or without silica gel. NO was measured after 0, 6, 9, 24 and 48 h. Scheffe F-tests were used to compare NO values. Results NO remained stable in balloons for 9 h at all temperatures, without silica gel. NO increased between 9 and 48 h, but only with low initial FENO. Silica gel increased variability.

Conclusions: FENO in mylar balloons is stable for at least 9 h. The storage temperature is not critical, but silica gel increases variability.