Mediators of Inflammation

Mediators of Inflammation / 2004 / Article

Open Access

Volume 13 |Article ID 181607 |

Habib Houman, Agnes Hamzaoui, Imed Ben Ghorbal, Monia Smiti Khanfir, Moncef Feki, Kamel Hamzaoui, "Tc1/Tc2 ratio in the inflammatory process in patients with Behçet's disease", Mediators of Inflammation, vol. 13, Article ID 181607, 7 pages, 2004.

Tc1/Tc2 ratio in the inflammatory process in patients with Behçet's disease


BACKGROUND: Peripheral blood CD8+ T cells expressing interferon gamma and interleukin-4 (IL-4), and lacking CD28 molecules, were responsible for the dynamic interplay between peripheral blood and inflammatory sites.Introduction: The aim of the current study was to define in Behçet's disease (BD), CD8+ T-cell subsets using CD28 and CD11b monoclonal antibodies, and the characterization of the Tc1/Tc2 ratio and perforin expression.Methods: Flow cytometry was used for intracytoplasmic cytokines and perforin expression. Effector cells were investigated by adhesion of CD8+ T cells to human microvascular endothelial cells and by chemotaxis using β-chemokine.Results: Interferon-gamma-producing CD8+ T cells in active and remission BD patients were increased, which induce a significant increase of the Tc1:Tc2 ratio in BD. CD8+CD28CD11b+ T cells were found to be more expanded in BD patients than in age-matched healthy controls. The expression of CD11b molecules in active BD allowed to CD8+CD28+/CD8+CD28 subsets to adhere to human microvascular endothelial cells, with more efficiency in BD. Using MIP-1α, we observed that the migratory process of CD28CD11b+ is more important in BD. CD28CD11b+ exhibited an increased perforin expression in BD patients.Conclusion: Taken together these results suggest the presence of immune activation, probably in response to a profound inflammation affecting BD patients. The physiopathological significance of these results were toward autoimmune diseases and/or infectious process.

Copyright © 2004 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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