PDF
Open Access
Hans Dieter Nischalke, Jacob Nattermann, Hans-Peter Fischer, Tilman Sauerbruch, Ulrich Spengler, Franz Ludwig Dumoulin, "Semiquantitative analysis of intrahepatic CC-chemokine mRNAs in chronic hepatitis C", Mediators of Inflammation, vol. 13, Article ID 651230, 3 pages, 2004. https://doi.org/10.1080/09629350400003159
Semiquantitative analysis of intrahepatic CC-chemokine mRNAs in chronic hepatitis C
Abstract
BACKGROUND: The mechanisms leading to hepatic injury in chronic hepatitis C virus (HCV) infection are only incompletely understood. Recent data propose a correlation of the intrahepatic expression of the CC chemokine RANTES and the degree of periportal and portal inflammatory liver damage.Aim: Here, we have studied the intrahepatic mRNA levels of CC chemokines RANTES together with that of other members of this chemokine family (MIP-1β, MCP-1, and MCP-2) in chronic hepatitis C as compared with healthy controls.Methods: Liver samples from 22 HCV-infected patients, nine individuals with primary biliary cirrhosis and from 12 normal controls were included into this study. Intrahepatic mRNA levels of CC chemokines RANTES, MIP-1β, MCP-1, and MCP-2 were analyzed by a semi-quantitative reverse transcription/real-time polymerase chain reaction assay.Results: In chronic HCV infection, intrahepatic RANTES mRNA levels were significantly higher than in non-infected controls (7.2-fold,
Copyright
Copyright © 2004 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
