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Mediators of Inflammation
Volume 2005, Issue 2, Pages 88-95

Proinflammatory Cytokines in Antilipopolysaccharide Immunity Against Klebsiella Infections

1Department of Microbiology and Parasitology, Medical Faculty, University of Rijeka, Brace Branchetta 20, Rijeka HR-51000, Croatia
2Department of Microbiology, Institute of Public Health, Kresimirova 52a, Rijeka HR-51000, Croatia

Received 5 November 2004; Accepted 20 December 2004

Copyright © 2005 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This study was undertaken in order to determine whether proinflammatory cytokines are involved in a previously described protection against Klebsiella infection mediated by antilipopolysaccharide antibodies. BALB/c mice were infected intraperitoneally with a lethal challenge of Klebsiella pneumoniae Caroli. One group of mice was protected with monoclonal antibodies against lipopolysaccharide prior to infection and the second was not. We determined the number of colony-forming units at different time points in the blood of infected animals and paralleled them with plasma levels of five proinflammatory cytokines measured by enzyme immunoassays. Our results show that the two groups of animals tested expressed different plasma concentrations for all cytokines. The greatest difference was detected 24 hours after infection, with a higher production in the unprotected group. We concluded that a reduced cytokine production is partially responsible for the survival of protected animals.