Flunisolide Decreases Exhaled Nitric Oxide and Nitrotyrosine Levels in Asthmatic Children

Bodini, A.; Peroni, D. G.; Zardini, F.; Corradi, M.; Alinovi, R.; Boner, A. L.; Piacentini, G. L.

doi:https://doi.org/10.1155/MI/2006/31919

Mediators of Inflammation

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Research Communication | Open Access

Volume 2006 | Article ID 031919 | https://doi.org/10.1155/MI/2006/31919

Flunisolide Decreases Exhaled Nitric Oxide and Nitrotyrosine Levels in Asthmatic Children

A. Bodini,¹D. G. Peroni,¹F. Zardini,¹M. Corradi,²R. Alinovi,²A. L. Boner,¹and G. L. Piacentini¹

Received10 Apr 2006

Revised05 Jun 2006

Accepted27 Jun 2006

Published27 Jul 2006

Abstract

Background. Exhaled nitric oxide (FeNO) has been reported to be elevated in the oxidative stress involved in asthmatic patients, and the reaction of nitric oxide (NO) with superoxide anions results in the formation of nitrotyrosine. The purpose of this study was to investigate the effect of inhaled steroid treatment on nitrotyrosine levels collected by exhaled breath condensate (EBC) and on FeNO. Methods. This was a single-blind placebo-controlled study. The lung function, FeNO, and nitrotyrosine levels were evaluated in 10 asthmatic children. Results. The nitrotyrosine levels were stable during the placebo period (T0 = 1.16 ng/ml versus T1 = 1.05 ng/ml; NS.), whereas they decreased after the treatment with flunisolide (T2 = 1.14 ng/ml versus T3 = 0.88 ng/ml; P<.001). No significant reduction in FeNO levels was observed after placebo treatment (T0 = 38.4 ppb versus T1 = 34.7 ppb, NS.). In contrast, FeNO values decreased significantly being at T3 = 14.9 ppb (T1 versus T3; P=.024). Conclusions. This study shows that corticosteroid treatment reduces nitrotyrosine levels in EBC of asthmatic subjects.

References

I Rahman, D Morrison, K Donaldson, and W Macnee, “Systemic oxidative stress in asthma, COPD, and smokers,” American Journal of Respiratory and Critical Care Medicine, vol. 154, no. 4 I, pp. 1055–1060, 1996.
View at: Google Scholar
T Hanazawa, S A Kharitonov, and P J Barnes, “Increased nitrotyrosine in exhaled breath condensate of patients with asthma,” American Journal of Respiratory and Critical Care Medicine, vol. 162, no. 4 I, pp. 1273–1276, 2000.
View at: Google Scholar
J S Beckman, T W Beckman, J Chen, P A Marshall, and B A Freeman, “Apparent hydroxyl radical production by peroxynitrite: implications for endothelial injury from nitric oxide and superoxide,” Proceedings of the National Academy of Sciences of the United States of America, vol. 87, no. 4, pp. 1620–1624, 1990.
View at: Google Scholar
S Matalon, P Hu, H Ischiropoulos, and J S Beckman, “Peroxynitrite inhibition of oxygen consumption and ion transport in alveolar type II pneumocytes,” Chest, vol. 105, no. 3 suppl, p. 74S, 1994.
View at: Google Scholar
G Sadeghi-Hashjin, G Folkerts, P AJ Henricks et al., “Peroxynitrite induces airway hyperresponsiveness in guinea pigs in vitro and in vivo,” American Journal of Respiratory and Critical Care Medicine, vol. 153, no. 5, pp. 1697–1701, 1996.
View at: Google Scholar
P Montuschi, M Corradi, G Ciabattoni, J Nightingale, S A Kharitonov, and P J Barnes, “Increased 8-isoprostane, a marker of oxidative stress, in exhaled condensate of asthma patients,” American Journal of Respiratory and Critical Care Medicine, vol. 160, no. 1, pp. 216–220, 1999.
View at: Google Scholar
P J Barnes and S A Kharitonov, “Exhaled nitric oxide: a new lung function test,” Thorax, vol. 51, no. 3, pp. 233–237, 1996.
View at: Google Scholar
J Mattes, K Storm Van's Gravesande, U Reining et al., “NO in exhaled air is correlated with markers of eosinophilic airway inflammation in corticosteroid-dependent childhood asthma,” European Respiratory Journal, vol. 13, no. 6, pp. 1391–1395, 1999.
View at: Publisher Site | Google Scholar
D Saleh, P Ernst, S Lim, P J Barnes, and A Giaid, “Increased formation of the potent oxidant peroxynitrite in the airways of asthmatic patients is associated with induction of nitric oxide synthase: effect of inhaled glucocorticoid,” FASEB Journal, vol. 12, no. 11, pp. 929–937, 1998.
View at: Google Scholar
E Baraldi, S Carraro, R Alinovi et al., “Cysteinyl leukotrienes and 8-isoprostane in exhaled breath condensate of children with asthma exacerbations,” Thorax, vol. 58, no. 6, pp. 505–509, 2003.
View at: Publisher Site | Google Scholar
“American Thoracic Society Committee on Diagnostic Standards for Nontubercolosis Respiratory Diseases. Definitions and classifications of chronic bronchitis, asthma and pulmonary emphysema,” American Review of Respiratory Disease, vol. 85, pp. 762–768, 1962.
View at: Google Scholar
“American Thoracic Society. Standardization of spirometry: 1994 update,” American Journal of Respiratory and Critical Care Medicine, vol. 152, no. 3, pp. 1107–1136, 1995.
View at: Google Scholar
E Baraldi and J de Jongste, “European Respiratory Society / American Thoracic Society. Measurement of exhaled nitric oxide in children, 2001,” European Respiratory Journal, vol. 20, no. 1, pp. 223–237, 2002.
View at: Google Scholar
J Bousquet, P K Jeffery, W W Busse, M Johnson, and A M Vignola, “Asthma from bronchoconstriction to airway inflammation and remodelling,” American Journal of Respiratory and Critical Care Medicine, vol. 161, no. 5, pp. 1720–1745, 2000.
View at: Google Scholar
S A Kharitonov, D H Yates, R A Robbins, R Logan-Sinclair, E A Shinebourne, and P J Barnes, “Increased nitric oxide in exhaled air of asthmatic patients,” Lancet, vol. 15, no. 343, pp. 133–135, 1994.
View at: Google Scholar
A Jatakanon, S Kharitonov, S Lim, and P J Barnes, “Effect of differing doses of inhaled budesonide on markers of airway inflammation in patients with mild asthma,” Thorax, vol. 54, no. 2, pp. 108–114, 1999.
View at: Google Scholar
P G Gibson, R L Henry, and P Thomas, “Noninvasive assessment of airway inflammation in children: induced sputum, exhaled nitric oxide, and breath condensate,” European Respiratory Journal, vol. 16, no. 5, pp. 1008–1015, 2000.
View at: Google Scholar
B Balint, S A Kharitonov, T Hanazawa et al., “Increased nitrotyrosine in exhaled breath condensate in cystic fibrosis,” European Respiratory Journal, vol. 17, no. 6, pp. 1201–1207, 2001.
View at: Publisher Site | Google Scholar
D A Kaminsky, J Mitchell, N Carroll, A James, R Soultanakis, and Y Janssen, “Nitrotyrosine formation in the airways and lung parenchyma of patients with asthma,” Journal of Allergy and Clinical Immunology, vol. 104, no. 4 I, pp. 747–754, 1999.
View at: Google Scholar
E Baraldi, G Giordano, M F Pasquale et al., “3-Nitrotyrosine, a marker of nitrosative stress, is increased in breath condensate of allergic asthmatic children,” Allergy: European Journal of Allergy and Clinical Immunology, vol. 61, no. 1, pp. 90–96, 2006.
View at: Publisher Site | Google Scholar
C Bergeron, H P Hauber, M Gotfried et al., “Evidence of remodeling in peripheral airways of patients with mild to moderate asthma: effect of hydrofluoroalkane-flunisolide,” Journal of Allergy and Clinical Immunology, vol. 116, no. 5, pp. 983–989, 2005.
View at: Publisher Site | Google Scholar

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Copyright © 2006 A. Bodini et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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