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Mediators of Inflammation
Volume 2006, Article ID 73835, 6 pages
http://dx.doi.org/10.1155/MI/2006/73835
Research Communication

Regulation of Annexin I in Rheumatoid Synovial Cells by Glucocorticoids and Interleukin-1

Centre for Inflammatory Diseases, Monash Institute for Medical Research, Monash Medical Centre, Locked Bag No 29, Clayton, Victoria 3168, Australia

Received 6 January 2006; Revised 23 January 2006; Accepted 23 January 2006

Copyright © 2006 Eric F. Morand et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The glucocorticoid (GC)-induced antiinflammatory molecule annexin I is expressed in leukocytes and has antiinflammatory effects in animal models of arthritis, but the expression of annexin I in rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLS) is unknown. We report the constitutive and dexamethasone (DEX)-inducible expression of annexin I in RA FLS. DEX increased FLS annexin I protein translocation and mRNA expression. Interleukin (IL)-1β also induced annexin I translocation and mRNA but also increased intracellular protein. DEX and IL-1 had additive effects on annexin I mRNA, but DEX inhibited the inducing effect of IL-1β on cell surface annexin I. These results indicate that glucocorticoids and IL-1β upregulate the synthesis and translocation of annexin I in RA FLS, but interdependent signalling pathways are involved.