Research Article | Open Access
Alexandra Bukovská, Štefan Cikoš, Štefan Juhás, Gabriela Il’ková, Pavol Rehák, Juraj Koppel, "Effects of a Combination of Thyme and Oregano Essential Oils on TNBS-Induced Colitis in Mice", Mediators of Inflammation, vol. 2007, Article ID 023296, 9 pages, 2007. https://doi.org/10.1155/2007/23296
Effects of a Combination of Thyme and Oregano Essential Oils on TNBS-Induced Colitis in Mice
We examined the anti-inflammatory effects of the combination of thyme and oregano essential oil dietary administered at three concentrations (0.4% thyme and 0.2% oregano oils; 0.2% thyme and 0.1% oregano oils; 0.1% thyme and 0.05% oregano oils) on mice with TNBS-induced colitis. Treatment of colitic animals with the essential oils decreased the mRNA levels of pro-inflammatory cytokines IL-1, IL-6, GM-CSF, and TNF, especially after application of the medium dose. The medium dose of the essential oils significantly lowered the amount of IL-1 and IL-6 proteins too. Moreover, administration of the medium dose decreased the mortality rate, accelerated the body weight gain recovery, and reduced the macroscopic damage of the colonic tissue. Our results indicate that combined treatment with appropriate concentrations of thyme and oregano essential oils can reduce the production of proinflammatory cytokines, and thereby attenuate TNBS-induced colitis in mice.
- J. Panés, “Inflammatory bowel disease: pathogenesis and targets for therapeutic interventions,” Acta Physiologica Scandinavica, vol. 173, no. 1, pp. 159–165, 2001.
- Y. H. Kho, M. Oudkerk Pool, F. G. A. Jansman, and J. W. Harting, “Pharmacotherapeutic options in inflammatory bowel disease: an update,” Pharmacy World & Science, vol. 23, no. 1, pp. 17–21, 2001.
- T. Hong, G.-B. Jin, G. Yoshino et al., “Protective effects of Polygalae root in experimental TNBS-induced colitis in mice,” Journal of Ethnopharmacology, vol. 79, no. 3, pp. 341–346, 2002.
- K. Sugimoto, H. Hanai, K. Tozawa et al., “Curcumin prevents and ameliorates trinitrobenzene sulfonic acid-induced colitis in mice,” Gastroenterology, vol. 123, no. 6, pp. 1912–1922, 2002.
- S. Maity, A. Ukil, S. Karmakar et al., “Thearubigin, the major polyphenol of black tea, ameliorates mucosal injury in trinitrobenzene sulfonic acid-induced colitis,” European Journal of Pharmacology, vol. 470, no. 1-2, pp. 103–112, 2003.
- A. Ukil, S. Maity, S. Karmakar, N. Datta, J. R. Vedasiromoni, and P. K. Das, “Curcumin, the major component of food flavour turmeric, reduces mucosal injury in trinitrobenzene sulphonic acid-induced colitis,” British Journal of Pharmacology, vol. 139, no. 2, pp. 209–218, 2003.
- K. H. Kwon, A. Murakami, T. Tanaka, and H. Ohigashi, “Dietary rutin, but not its aglycone quercetin, ameliorates dextran sulfate sodium-induced experimental colitis in mice: attenuation of pro-inflammatory gene expression,” Biochemical Pharmacology, vol. 69, no. 3, pp. 395–406, 2005.
- S. V. Popov, R. G. Ovodova, P. A. Markov, I. R. Nikitina, and Y. S. Ovodov, “Protective effect of comaruman, a pectin of cinquefoil Comarum palustre L., on acetic acid-induced colitis in mice,” Digestive Diseases and Sciences, vol. 51, no. 9, pp. 1532–1537, 2006.
- Y.-H. Zhou, J.-P. Yu, Y.-F. Liu et al., “Effects of Ginkgo biloba extract on inflammatory mediators (SOD, MDA, TNF-, NF-Bp65, IL-6) in TNBS-induced colitis in rats,” Mediators of Inflammation, vol. 2006, Article ID 92642, 9 pages, 2006.
- G. Rogler and T. Andus, “Cytokines in inflammatory bowel disease,” World Journal of Surgery, vol. 22, no. 4, pp. 382–389, 1998.
- S. Wirtz and M. F. Neurath, “Animal models of intestinal inflammation: new insights into the molecular pathogenesis and immunotherapy of inflammatory bowel disease,” International Journal of Colorectal Disease, vol. 15, no. 3, pp. 144–160, 2000.
- P. L. Bertevello, A. F. Logullo, S. Nonogaki et al., “Immunohistochemical assessment of mucosal cytokine profile in acetic acid experimental colitis,” Clinics, vol. 60, no. 4, pp. 277–286, 2005.
- D. Raddatz, M. Bockemühl, and G. Ramadori, “Quantitative measurement of cytokine mRNA in inflammatory bowel disease: relation to clinical and endoscopic activity and outcome,” European Journal of Gastroenterology & Hepatology, vol. 17, no. 5, pp. 547–557, 2005.
- H. M. Alzuherri, C. J. Woodall, and C. J. Clarke, “Increased intestinal TNF-, IL-1 and IL-6 expression in ovine paratuberculosis,” Veterinary Immunology and Immunopathology, vol. 49, no. 4, pp. 331–345, 1996.
- S. Fournout, C. M. Dozois, M. Odin et al., “Lack of a role of cytotoxic necrotizing factor 1 toxin from Escherichia coli in bacterial pathogenicity and host cytokine response in infected germfree piglets,” Infection and Immunity, vol. 68, no. 2, pp. 839–847, 2000.
- T. Brasseur, “Etudes botaniques, phytochimiques et pharmacologiques consacrées au thym,” Journal de Pharmacie de Belgique, vol. 38, no. 5, pp. 261–272, 1983.
- T. Essawi and M. Srour, “Screening of some Palestinian medicinal plants for antibacterial activity,” Journal of Ethnopharmacology, vol. 70, no. 3, pp. 343–349, 2000.
- K. Miura, H. Kikuzaki, and N. Nakatani, “Antioxidant activity of chemical components from sage (Salvia officinalis L.) and thyme (Thymus vulgaris L.) measured by the oil stability index method,” Journal of Agricultural and Food Chemistry, vol. 50, no. 7, pp. 1845–1851, 2002.
- K. M. Soliman and R. I. Badeaa, “Effect of oil extracted from some medicinal plants on different mycotoxigenic fungi,” Food and Chemical Toxicology, vol. 40, no. 11, pp. 1669–1675, 2002.
- M. Hudaib, E. Speroni, A. M. Di Pietra, and V. Cavrini, “GC/MS evaluation of thyme (Thymus vulgaris L.) oil composition and variations during the vegetative cycle,” Journal of Pharmaceutical and Biomedical Analysis, vol. 29, no. 4, pp. 691–700, 2002.
- P. C. Braga, M. Dal Sasso, M. Culici, T. Bianchi, L. Bordoni, and L. Marabini, “Anti-inflammatory activity of thymol: inhibitory effect on the release of human neutrophil elastase,” Pharmacology, vol. 77, no. 3, pp. 130–136, 2006.
- Y. Suzuki and H. Furuta, “Stimulation of guinea pig neutrophil superoxide anion-producing system with thymol,” Inflammation, vol. 12, no. 6, pp. 575–584, 1988.
- R. Aeschbach, J. Löliger, B. C. Scott et al., “Antioxidant actions of thymol, carvacrol, 6-gingerol, zingerone and hydroxytyrosol,” Food and Chemical Toxicology, vol. 32, no. 1, pp. 31–36, 1994.
- N. Didry, L. Dubreuil, and M. Pinkas, “Activity of thymol, carvacrol, cinnamaldehyde and eugenol on oral bacteria,” Pharmaceutica Acta Helvetiae, vol. 69, no. 1, pp. 25–28, 1994.
- M. E. Venturini, D. Blanco, and R. Oria, “In vitro antifungal activity of several antimicrobial compounds against Penicillium expansum,” Journal of Food Protection, vol. 65, no. 5, pp. 834–839, 2002.
- A. Mahmoud, “Antifungal action and anti aflatoxigenic properties of some essential oil constituents,” Letters in Applied Microbiology, vol. 19, pp. 110–113, 1994.
- S. Fujisawa and Y. Kadoma, “Effect of phenolic compounds on the polymerization of methyl methacrylate,” Dental Materials, vol. 8, no. 5, pp. 324–326, 1992.
- M. H. Alma, A. Mavi, A. Yildirim, M. Digrak, and T. Hirata, “Screening chemical composition and in vitro antioxidant and antimicrobial activities of the essential oils from Origanum syriacum L. growing in Turkey,” Biological & Pharmaceutical Bulletin, vol. 26, no. 12, pp. 1725–1729, 2003.
- C. C. Arcila-Lozano, G. Loarca-Piña, S. Lecona-Uribe, and E. González De Mejía, “Oregano: properties, composition and biological activity,” Archivos Latinoamericanos de Nutricion, vol. 54, no. 1, pp. 100–111, 2004.
- H. Tian and D. M. Lai, “Analysis on the volatile oil in Origanum vulgare,” Zhongyaocai, vol. 29, no. 9, pp. 920–921, 2006.
- B. Bozin, N. Mimica-Dukic, N. Simin, and G. Anackov, “Characterization of the volatile composition of essential oils of some lamiaceae spices and the antimicrobial and antioxidant activities of the entire oils,” Journal of Agricultural and Food Chemistry, vol. 54, no. 5, pp. 1822–1828, 2006.
- K. Horošová, D. Bujňáková, and V. Kmet, “Effect of oregano essential oil on chicken Lactobacilli and E. coli,” Folia Microbiologica, vol. 51, no. 4, pp. 278–280, 2006.
- J. L. Wallace, W. K. MacNaughton, G. P. Morris, and P. L. Beck, “Inhibition of leukotriene synthesis markedly accelerates healing in a rat model of inflammatory bowel disease,” Gastroenterology, vol. 96, no. 1, pp. 29–36, 1989.
- Š. Juhás, Š. Cikoš, S. Czikková et al., “Effects of borneol and thymoquinone on TNBS induced colitis in mice,” submitted to Folia Biologica.
- J. Vandesompele, K. De Preter, F. Pattyn et al., “Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes,” Genome Biology, vol. 3, no. 7, pp. research0034.1–research0034.11, 2002.
- M. M. Bradford, “A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding,” Analytical Biochemistry, vol. 72, no. 1-2, pp. 248–254, 1976.
- A. A. Parikh, A. L. Salzman, J. E. Fischer, C. Szabó, and P.-O. Hasselgren, “Interleukin-1 and interferon- regulate interleukin-6 production in cultured human intestinal epithelial cells,” Shock, vol. 8, no. 4, pp. 249–255, 1997.
- E. S. Hungness, T. A. Pritts, G.-J. Luo, X. Sun, C. G. Penner, and P.-O. Hasselgren, “The transcription factor activator protein-1 is activated and interleukin-6 production is increased in interleukin-1 -stimulated human enterocytes,” Shock, vol. 14, no. 3, pp. 386–391, 2000.
- S. Aydin, A. A. Başaran, and N. Başaran, “Modulating effects of thyme and its major ingredients on oxidative DNA damage in human lymphocytes,” Journal of Agricultural and Food Chemistry, vol. 53, no. 4, pp. 1299–1305, 2005.
- F. Dušan, S. Marián, D. Katarína, and B. Dobroslava, “Essential oils—their antimicrobial activity against Escherichia coli and effect on intestinal cell viability,” Toxicology in Vitro, vol. 20, no. 8, pp. 1435–1445, 2006.
- S. Santoyo, S. Cavero, L. Jaime, E. Ibañez, F. J. Señoráns, and G. Reglero, “Supercritical carbon dioxide extraction of compounds with antimicrobial activity from Origanum vulgare L.: determination of optimal extraction parameters,” Journal of Food Protection, vol. 69, no. 2, pp. 369–375, 2006.
- B. Adam, T. Liebregts, J. Best et al., “A combination of peppermint oil and caraway oil attenuates the post-inflammatory visceral hyperalgesia in a rat model,” Scandinavian Journal of Gastroenterology, vol. 41, no. 2, pp. 155–160, 2006.
- Z. Sun and R. Andersson, “NF-B activation and inhibition: a review,” Shock, vol. 18, no. 2, pp. 99–106, 2002.
- Y. Deguchi, A. Andoh, O. Inatomi et al., “Curcumin prevents the development of dextran sulfate sodium (DSS)-induced experimental colitis,” to apper in Digestive Diseases and Sciences.
- A. Murakami, K. Matsumoto, K. Koshimizu, and H. Ohigashi, “Effects of selected food factors with chemopreventive properties on combined lipopolysaccharide- and interferon--induced IB degradation in RAW264.7 macrophages,” Cancer Letters, vol. 195, no. 1, pp. 17–25, 2003.
- Y. L. Lin, S. H. Tsai, S. Y. Lin-Shiau, C. T. Ho, and J. K. Lin, “Theaflavin-3,-digallate from black tea blocks the nitric oxide synthase by down-regulating the activation of NF-B in macrophages,” European Journal of Pharmacology, vol. 367, no. 2-3, pp. 379–388, 1999.
- K. Stalińska, A. Guzdek, M. Rokicki, and A. Koj, “Transcription factors as targets of the anti-inflammatory treatment. A cell culture study with extracts from some Mediterranean diet plants,” Journal of Physiology and Pharmacology, vol. 56, supplement 1, pp. 157–169, 2005.
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