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Mediators of Inflammation
Volume 2007, Article ID 30987, 4 pages
http://dx.doi.org/10.1155/2007/30987
Research Article

Antimicrobial Activity Does Not Predict Cytokine Response to Adrenomedullin or Its Shortened Derivatives

1Institute of Dentistry, Queen Mary, University of London, Newark Street, London E1 2AT, UK
2Department of Asthma, Allergy and Respiratory Science, Kings College London, 5th Floor, Thomas Guy House, Guy's Hospital, London SE1 9RT, UK

Received 18 June 2007; Revised 9 July 2007; Accepted 5 September 2007

Copyright © 2007 Quratul Ann Hussain et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of this study was to investigate cytokine release from oral keratinocytes and fibroblasts in response to AM and shortened derivatives previously characterised in terms of their antimicrobial activities. Cells were incubated with AM or its fragments (residues 1-12, 1-21, 13-52, 16-21, 16-52, 22-52, 26-52, and 34-52), and culture supernatants collected after 1, 2, 4, 8, and 24 hours. A time-dependant increase in production of interleukin1-α and interleukin 1-β from keratinocytes in response to all peptides was demonstrated. However, exposure to fragments compared to whole AM resulted in reduced production of these cytokines (60% mean reduction at 24 hours, P<.001). No consistent differences were shown between the cytokine response elicited by antimicrobial and nonantimicrobial fragments. The production of interleukin-6 and interleukin-8 did not change significantly with time or peptide used. Fibroblast cells were relatively unresponsive to all treatments. This study demonstrates that antimicrobial activity does not predict cytokine response to adrenomedullin or its shortened derivatives.