Tumor Necrosis Factor-Alpha in Peripical Tissue Exudates of Teeth with Apical Periodontitis

Pezelj-Ribaric, Sonja; Magašic, Karolina; Prpic, Jelena; Miletic, Ivana; Karlovic, Zoran

doi:https://doi.org/10.1155/2007/69416

Mediators of Inflammation

On this page

Abstract References Copyright Related Articles

Clinical Study | Open Access

Volume 2007 | Article ID 069416 | https://doi.org/10.1155/2007/69416

Tumor Necrosis Factor-Alpha in Peripical Tissue Exudates of Teeth with Apical Periodontitis

Sonja Pezelj-Ribaric,¹Karolina Magašic,¹Jelena Prpic,¹Ivana Miletic,²and Zoran Karlovic²

Received20 Sept 2007

Accepted04 Dec 2007

Published23 Jan 2008

Abstract

Aim. The aim of this study was to determine tumor necrosis factor-alpha (TNF-α) levels in periapical exudates and to evaluate their relationship with radiological findings. Methodology. Periapical exudates were collected from root canals of 60 single-rooted teeth using absorbent paper points. TNF-α levels were determined by enzyme-linked immunosorbent assays. The samples were divided into three groups according to the periapical radiolucent area. Results. Nonparametric Kruskal-Wallis test revealed significant differences between TNF-α concentrations in control group (40, 57±28, 15 pg/mL) and group with larger radiolucent areas (2365, 79±582, 95 pg/mL), as well as between control and canals with small radiolucent areas (507, 66±278, 97) (P<.05). Conclusions. The levels of TNF-α increase significantly in teeth with periapical pathosis, from smaller to bigger lesions. This research and its results have shown that objective analysis of the TNF-α levels enables establishment of a relationship between different concentrations of TNF-α and different radiological changes.

References

J. Danin, L. E. Linder, G. Lundqvist, and L. Andersson, “Tumor necrosis factor-alpha and transforming growth factor-beta1 in chronic periapical lesions,” Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics, vol. 90, no. 4, pp. 514–517, 2000.
View at: Publisher Site | Google Scholar
A. R. De Sá, F. J. G. S. Pimenta, W. O. Dutra, and R. S. Gomez, “Immunolocalization of interleukin 4, interleukin 6, and Iymphotoxin $α$ in dental granulomas,” Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics, vol. 96, no. 3, pp. 356–360, 2003.
View at: Publisher Site | Google Scholar
E. Girardin, P. Roux-Lombard, G. E. Grau, P. Suter, H. Gallati, and J. M. Dayer, “Imbalance between tumour necrosis factor-alpha and soluble TNF receptor concentrations in severe meningococcaemia. The J5 Study Group,” Immunology, vol. 76, no. 1, pp. 20–23, 1992.
View at: Google Scholar
H. Kabashima, K. Nagata, K. Maeda, and T. Iijima, “Involvement of substance P, mast cells, TNF- $α$ and ICAM-1 in the infiltration of inflammatory cells in human periapical granulomas,” Journal of Oral Pathology and Medicine, vol. 31, no. 3, pp. 175–180, 2002.
View at: Publisher Site | Google Scholar
J. R. Paysant, A. Rupin, and T. J. Verbeuren, “Effect of NADPH oxidase inhibition on E-selectin expression induced by concomitant anoxia/reoxygenation and TNF- $α$ ,” Endothelium, vol. 9, no. 4, pp. 263–271, 2002.
View at: Publisher Site | Google Scholar
T. Ataoglu, M. Ungor, B. Serpek, S. Haliloglu, H. Ataoglu, and H. Ari, “Interleukin-1 $β$ and tumour necrosis factor- $α$ levels in periapical exudates,” International Endodontic Journal, vol. 35, no. 2, pp. 181–185, 2002.
View at: Publisher Site | Google Scholar
H. Shimauchi, Y. Miki, S.-I. Takayama, T. Imai, and H. Okada, “Development of a quantitative sampling method for periapical exudates from human root canals,” Journal of Endodontics, vol. 22, no. 11, pp. 612–615, 1996.
View at: Publisher Site | Google Scholar
M.-L. Kuo, I. B. Lamster, and G. Hasselgren, “Host mediators in endodontic exudates. II. Changes in concentration with sequential sampling,” Journal of Endodontics, vol. 24, no. 10, pp. 636–640, 1998.
View at: Publisher Site | Google Scholar
M.-L. Kuo, I. B. Lamster, and G. Hasselgren, “Host mediators in endodontic exudates. I. Indicators of inflammation and humoral immunity,” Journal of Endodontics, vol. 24, no. 9, pp. 598–603, 1998.
View at: Publisher Site | Google Scholar
A. J. Delima, T. Oates, R. Assuma et al., “Soluble antagonists to interleukin-1 (IL-1) and tumor necrosis factor (TNF) inhibits loss of tissue attachment in experimental periodontitis,” Journal of Clinical Periodontology, vol. 28, no. 3, pp. 233–240, 2001.
View at: Publisher Site | Google Scholar
H. Shimauchi, S. Takayama, T. Imai-Tanaka, and H. Okada, “Balance of interleukin-1 $β$ and interleukin-1 receptor antagonist in human periapical lesions,” Journal of Endodontics, vol. 24, no. 2, pp. 116–119, 1998.
View at: Publisher Site | Google Scholar
M. Kjeldsen, P. Holmstrup, and K. Bendtzen, “Marginal periodontitis and cytokines: a review of literature,” Journal of Periodontology, vol. 26, pp. 366–370, 1993.
View at: Google Scholar
Y.-C. Chang, S.-F. Yang, F.-M. Huang, K.-W. Tai, and Y.-S. Hsieh, “Induction of tissue plasminogen activator gene expression by proinflammatory cytokines in human pulp and gingival fibroblasts,” Journal of Endodontics, vol. 29, no. 2, pp. 114–117, 2003.
View at: Publisher Site | Google Scholar
L. Artese, C. Rubini, G. Ferrero, and M. Fioroni, “Vascular endothelial growth factor (VEGF) expression in healthy and inflammatory pulps,” Journal of Endodontics, vol. 28, no. 1, pp. 20–23, 2002.
View at: Publisher Site | Google Scholar
C.-Y. Wang, N. Tani-Ishii, and P. Stashenko, “Bone-resorptive cytokine gene expression in periapical lesions in the rat,” Oral Microbiology and Immunology, vol. 12, no. 2, pp. 65–71, 1997.
View at: Google Scholar
S. Pezelj-Ribarić, I. Anić, I. Brekalo, I. Miletić, M. Hasan, and M. Š. Šoškić, “Detection of TNF-alpha in normal and inflammed human dental pulps,” Archives of Medical Research, vol. 33, pp. 482–484, 2002.
View at: Google Scholar

Copyright

Copyright © 2007 Sonja Pezelj-Ribarić et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PDF Download Citation Order printed copies

Views

457

Downloads

1018

Citations