Mediators of Inflammation

Mediators of Inflammation / 2007 / Article

Clinical Study | Open Access

Volume 2007 |Article ID 73635 | 7 pages | https://doi.org/10.1155/2007/73635

Evaluation of C-Reactive Protein, Endothelin-1, Adhesion Molecule(s), and Lipids as Inflammatory Markers in Type 2 Diabetes Mellitus Patients

Received23 Oct 2006
Revised16 Nov 2006
Accepted18 Nov 2006
Published07 Feb 2007

Abstract

This study compared lipids, the product of lipid peroxidation malondialdehyde (MDA), the acute phase reactant high sensitive C-reactive protein (hsCRP), endothelin-1 (ET-1), P-selectin, intercellular adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1 (VCAM-1) between healthy controls, subjects with ischemic heart disease (IHD) and type 2 diabetes mellitus (DM) subjects who did not perform coronary artery bypass graft (CABG) surgery as well as type 2 DM subjects who performed CABG. HbA1c, lipids, MDA, hsCRP, ET-1, P-selectin, ICAM-1, and VCAM-1 levels were significantly higher in the diabetic groups than in either healthy controls or IHD subjects. In the diabetic groups, there was a negative association among hsCRP and HDL-C. ET-1, ICAM-1 levels and TAG were positively correlated, as do the association between P-selectin, VCAM-1 and HbA1c%. Also a positive relation was found among hsCRP levels and ICAM-1, as well as MDA and ET-1. P-selectin and ICAM-1 were significantly positively correlated. This study indicates that increased level of oxidative stress marker, proinflammatory markers and their downstream effectors adhesion molecules occurs in type 2 DM.

References

  1. O. I. Stenina, “Regulation of vascular genes by glucose,” Current Pharmaceutical Design, vol. 11, no. 18, pp. 2367–2381, 2005. View at: Publisher Site | Google Scholar
  2. R. C. A. Tostes and M. N. Muscará, “Endothelin receptor antagonists: another potential alternative for cardiovascular diseases,” Current Drug Targets - Cardiovascular and Haematological Disorders, vol. 5, no. 4, pp. 287–301, 2005. View at: Publisher Site | Google Scholar
  3. P. A. Insel, S. Kornfeld, P. W. Majerus et al., “Blasts from the past,” Journal of Clinical Investigation, vol. 114, no. 8, pp. 1017–1033, 2004. View at: Publisher Site | Google Scholar
  4. M. Meydani, “Soluble adhesion molecules: surrogate markers of cardiovascular disease?” Nutrition Reviews, vol. 61, no. 2, pp. 63–68, 2003. View at: Publisher Site | Google Scholar
  5. G. Davì, A. Falco, and C. Patrono, “Lipid peroxidation in diabetes mellitus,” Antioxidants and Redox Signaling, vol. 7, no. 1-2, pp. 256–268, 2005. View at: Publisher Site | Google Scholar
  6. A. Ergul, J. S. Johansen, C. Strømhaug et al., “Vascular dysfunction of venous bypass conduits is mediated by reactive oxygen species in diabetes: role of endothelin-1,” Journal of Pharmacology and Experimental Therapeutics, vol. 313, no. 1, pp. 70–77, 2005. View at: Publisher Site | Google Scholar
  7. E. C. Abraham, T. A. Huff, N. D. Cope, J. B. Wilson Jr., E. D. Bransome Jr., and T. H. Huisman, “Determination of the glycosylated hemoglobins (Hb A1) with a new microcolumn procedure. Suitability of the technique for assessing the clinical management of diabetes mellitus,” Diabetes, vol. 27, no. 9, pp. 931–937, 1978. View at: Google Scholar
  8. P. Trinder, “Determination of blood glucose using an oxidase-peroxidase system with a non-carcinogenic chromogen,” Journal of Clinical Pathology, vol. 22, no. 2, pp. 158–161, 1969. View at: Google Scholar
  9. W. Richmond, “Preparation and properties of a cholesterol oxidase from Nocardia sp. and its application to the enzymatic assay of total cholesterol in serum,” Clinical Chemistry, vol. 19, no. 12, pp. 1350–1356, 1973. View at: Google Scholar
  10. J. Siedel, E. O. Hagele, J. Ziegenhorn, and A. W. Wahlefeld, “Reagent for the enzymatic determination of serum total cholesterol with improved lipolytic efficiency,” Clinical Chemistry, vol. 29, no. 6, pp. 1075–1080, 1983. View at: Google Scholar
  11. M. Burstein, H. R. Scholnick, and R. Morfin, “Rapid method for the isolation of lipoproteins from human serum by precipitation with polyanions,” Journal of Lipid Research, vol. 11, no. 6, pp. 583–595, 1970. View at: Google Scholar
  12. W. T. Friedewald, R. I. Levy, and D. S. Fredrickson, “Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge,” Clinical Chemistry, vol. 18, no. 6, pp. 499–502, 1972. View at: Google Scholar
  13. J. I. Cleeman, “Executive summary of the third report of the National Cholesterol Education Program (NCEP) expert panel on detection, evaluation, and treatment of high blood cholesterol in adults (adult treatment panel III),” Journal of the American Medical Association, vol. 285, no. 19, pp. 2486–2497, 2001. View at: Publisher Site | Google Scholar
  14. D. R. Janero, “Malondialdehyde and thiobarbituric acid-reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury,” Free Radical Biology and Medicine, vol. 9, no. 6, pp. 515–540, 1990. View at: Publisher Site | Google Scholar
  15. D. G. Waller, A. G. Renwick, and K. Hillier, “Ischaemic heart disease,” in Medical Pharmacology and Therapeutics, p. 81, W. B. Saunders, Philadelphia, Pa, USA, 2001, chapter 5. View at: Google Scholar
  16. C. Vitale, M. Fini, F. Leonardo et al., “Effect of estradiol valerate alone or in association with cyproterone acetate upon vascular function of postmenopausal women at increased risk for cardiovascular disease,” Maturitas, vol. 40, no. 3, pp. 239–245, 2001. View at: Publisher Site | Google Scholar
  17. K. Matsumoto, Y. Sera, Y. Abe et al., “High serum concentrations of soluble E-selectin correlate with obesity but not fat distribution in patients with type 2 diabetes mellitus,” Metabolism, vol. 51, no. 7, pp. 932–934, 2002. View at: Publisher Site | Google Scholar
  18. N. Kaul, S. Devaraj, and I. Jialal, “α-tocopherol and atherosclerosis,” Proceedings of the Society for Experimental Biology and Medicine, vol. 226, no. 1, pp. 5–12, 2001. View at: Google Scholar
  19. F. O. Pereira, T. S. Frode, and Y. S. Medeiros, “Evaluation of tumour necrosis factor alpha, interleukin-2 soluble receptor, nitric oxide metabolites, and lipids as inflammatory markers in type 2 diabetes mellitus,” Mediators of Inflammation, vol. 2006, Article ID 39062, p. 7, 2006. View at: Publisher Site | Google Scholar
  20. M. B. Schulze, E. B. Rimm, T. Li, N. Rifai, M. J. Stampfer, and F. B. Hu, “C-reactive protein and incident cardiovascular events among men with diabetes,” Diabetes Care, vol. 27, no. 4, pp. 889–894, 2004. View at: Google Scholar
  21. A. Saitta, D. Altavilla, D. Cucinotta et al., “Randomized, double-blind, placebo-controlled study on effects of raloxifene and hormone replacement therapy on plasma NO concentrations, endothelin-1 levels, and endothelium-dependent vasodilation in postmenopausal women,” Arteriosclerosis, Thrombosis, and Vascular Biology, vol. 21, no. 9, pp. 1512–1519, 2001. View at: Google Scholar
  22. E. Ercan, H. Bozdemir, I. Tengiz et al., “Decreased soluble cell adhesion molecules after tirofiban infusion in patients with unstable angina pectoris,” Thrombosis Journal, vol. 2, no. 1, pp. 4–9, 2004. View at: Publisher Site | Google Scholar
  23. L. Calabresi, M. Gomaraschi, B. Villa, L. Omoboni, C. Dmitrieff, and G. Franceschini, “Atherosclerosis and Lipoproteins: elevated soluble cellular adhesion molecules in subjects with low HDL-cholesterol,” Arteriosclerosis, Thrombosis, and Vascular Biology, vol. 22, no. 4, pp. 656–661, 2002. View at: Publisher Site | Google Scholar
  24. T. Kerner, O. Ahlers, H. Reschreiter, C. Bührer, M. Möckel, and H. Gerlach, “Adhesion molecules in different treatments of acute myocardial infarction,” Critical Care, vol. 5, no. 3, pp. 145–150, 2001. View at: Publisher Site | Google Scholar
  25. N. Zimmermann, A. Wenk, U. Kim et al., “Functional and biochemical evaluation of platelet aspirin resistance after coronary artery bypass surgery,” Circulation, vol. 108, no. 5, pp. 542–547, 2003. View at: Publisher Site | Google Scholar
  26. I. Kowalska, M. Straczkowski, M. Szelachowska et al., “Circulating E-selectin, vascular cell adhesion molecule-1, and intercellular adhesion molecule-1 in men with coronary artery disease assessed by angiography and disturbances of carbohydrate metabolism,” Metabolism, vol. 51, no. 6, pp. 733–736, 2002. View at: Publisher Site | Google Scholar

Copyright © 2007 Hala El-Mesallamy et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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