Mycoplasma genitalium Lipoproteins Induce Human Monocytic Cell Expression of Proinflammatory Cytokines and Apoptosis by Activating Nuclear Factor B
Figure 3
The effect of
different groups on the expression of TNF- (a), IL-6 (b), and
IL-1 (c) mRNA. THP-1 cells were stimulated for 18 hours and the mRNA levels were
determined by RT-PCR. The levels of mRNA expression were inhibited upon the
introduction of 25 M PDTC. The bottom photograph depicts the result of RT-PCR
for mRNA, and the top photograph depicts the -actin positive control. Lane M:
100 bp DNA marker; lane 1: 0.1 g/mL LPS; lane 2: 3 g/mL of LP; lane 3: 3 g/mL
of LP in combination with 25 M PDTC; lane 4: 100 L aqueous phase (negative control).
