(-)-Epigallocatechin Gallate Reduces Platelet-Derived Growth Factor-BB-Stimulated Interleukin-6 Synthesis in Osteoblasts: Suppression of SAPK/JNK
Figure 4
Effect of EGCG on the phosphorylation of
SAPK/JNK induced by PDGF-BB in MC3T3-E1 cells. The cultured cells were
pretreated with the indicated doses of EGCG or vehicle for 60 minutes, and then
stimulated by 50 ng/mL PDGF-BB or vehicle for 3 minutes. The extracts of cells
were subjected to SDS-PAGE with subsequent Western blotting analysis with
antibodies against phospho-specific SAPK/JNK or SAPK/JNK. The histogram shows quantitative
representations of the levels of PDGF-BB-induced phosphorylation obtained from
laser densitometric analysis of three independent experiments. Each value
represents the meanSEM of triplicate determinations. Similar results were obtained with two
additional and different cell preparations. : compared to the value of
PDGF-BB alone.