(-)-Epigallocatechin Gallate Reduces Platelet-Derived Growth Factor-BB-Stimulated Interleukin-6 Synthesis in Osteoblasts: Suppression of SAPK/JNK
Figure 5
Effect of EGCG on the
phosphorylation of Akt and p70 S6 kinase induced by PDGF-BB in MC3T3-E1 cells.
The cultured cells were pretreated with the indicated doses of EGCG or vehicle
for 60 minutes, and then stimulated by 50 ng/mL PDGF-BB or vehicle for 20 minutes.
The extracts of cells were subjected to SDS-PAGE with subsequent Western
blotting analysis with antibodies against (a) phospho-specific Akt or Akt, and
(b) phospho-specific p70 S6 kinase or p70 S6 kinase. The histogram shows
quantitative representations of the levels of PDGF-BB-induced phosphorylation
obtained from laser densitometric analysis of three independent experiments.
Each value represents the meanSEM of triplicate determinations. Similar
results were obtained with two additional and different cell preparations.
