Suppressive Effect of Hydroquinone, a Benzene Metabolite, on In Vitro Inflammatory Responses Mediated by Macrophages, Monocytes, and Lymphocytes
Figure 2
Effect of hydroquinone on the production of cytokines and
their mRNA expression in LPS-activated RAW264.7 cells.
(a) RAW264.7
cells ( cells/mL) were incubated with indicated concentrations
of HQ (hydroquinone) in the presence or absence of LPS
(2 g/mL)
for 12 hours. Secreted levels of TNF-α, IL-1β,
and IL-6 in culture supernatant were determined by ELISA. (b) The mRNA levels of
proinflammatory cytokines from the RAW264.7 cells were determined by
semiquantitative RT-PCR. The
results show one representative experiment of three. and represent significant
difference as compared to control.
