Suppressive Effect of Hydroquinone, a Benzene Metabolite, on In Vitro Inflammatory Responses Mediated by Macrophages, Monocytes, and Lymphocytes
Figure 8
Effect of hydroquinone
on cell-cell or cell-fibronectin adhesion events and surface levels of
integrins (CD18 and CD29). (a) and (b) U937 cells ()
were incubated with indicated
concentrations of HQ (hydroquinone) in the
presence or absence of a proaggregation (activating) antibody against CD29
(MEM101A (1 μg/mL)) for
2 hours. The extent of cell-cell aggregation was determined by quantitative
cell-cell adhesion assay as described in Materials and Methods. (b) Images of cells in culture were obtained using an inverted phase contrast
microscope attached to a video camera. (c) U937 cells pretreated with
hydroquinone were seeded on fibronectin
(50 g/mL)-coated
plates and further incubated for 3 hours. Attached cells were determined by
crystal violet assay, as
described in Section 2. (d) U937 cells were incubated with hydroquinone (12.5 M) for 3 hours.
The surface expression
of adhesion molecules (CD18 and CD29) was determined by flow cytometric
analysis as described in Section 2. represents significant difference as compared to control.
