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Mediators of Inflammation
Volume 2008, Article ID 309721, 6 pages
http://dx.doi.org/10.1155/2008/309721
Research Article

Association of Single Nucleotide Polymorphisms in the IL-18 Gene with Production of IL-18 Protein by Mononuclear Cells from Healthy Donors

1Laboratory of Molecular Immunology, Institute of Clinical Immunology, Siberian Branch of Russian Academy of Medical Sciences (SB RAMS), Novosibirsk 630099, Russia
2Pharmacogenomic Group, Institute of Chemical Biology and Fundamental Medicine, Novosibirsk 630090, Russia

Received 24 June 2008; Accepted 5 September 2008

Academic Editor: Magdalena Klink

Copyright © 2008 O. P. Khripko et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

IL-18 has proinflammatory effects and participates in both innate and adaptive cellular and humoral immunity. A number of SNPs that influence IL-18 production are found in the gene promoter region. We investigated the association of SNPs in the IL-18 promoter at 607 and 137 with the level of IL-18 protein production by PBMC from healthy donors from Southwestern Siberia. The genetic distribution of these SNPs in the promoter site was established by PCR. IL-18 protein production was determined by ELISA. Our results showed that PBMC from donors carrying allele 137C have lower levels of both spontaneous and LPS-stimulated IL-18 production. In contrast, PBMC from donors carrying allele 607A showed significant increases in spontaneous and stimulated IL-18 production compared to wild type. Our study suggests that the SNPs 607 and 137 in the promoter region of the IL-18 gene influence the level of IL-18 protein production by PBMC from healthy donors in Southwestern Siberia.