The NF-B Inhibitor Curcumin Blocks Sepsis-Induced Muscle Proteolysis
Figure 2
Sepsis-induced expression of atrogin-1 in extensor digitorum longus muscles is not influenced by curcumin. Sham-operated and septic rats were treated with vehicle (control) or curcumin (total dose 1800 mg/kg divided into three equal doses administered intraperitoneally 1 hour before and 8 and 15 hours after sham-operation or CLP). Muscles were harvested 16 hours after sham-operation or CLP for (a) atrogin-1 mRNA levels determined by real-time PCR and (b) atrogin-1 protein levels determined by Western blotting. In panel (b), a representative Western blot is shown in the upper portion and results from densitometric quantifications are shown in the lower portion of the figure. Results are means ±SEM with in each group. AU: arbitrary units. * versus corresponding sham group by ANOVA.
