The NF-B Inhibitor Curcumin Blocks Sepsis-Induced Muscle Proteolysis
Figure 5
Treatment of incubated muscles in vitro with curcumin reduces sepsis-induced increase in protein breakdown. (a) Extensor digitorum longus muscles were harvested from rats 16 hours after sham-operation or CLP and incubated for 2 hours in the absence or presence of curcumin (100 M) dissolved in 0.1% DMSO. Protein breakdown rates were measured as net release of tyrosine as described in Section 2. Results are means ±SEM with in each group. * versus all other groups by ANOVA. (b) Incubated muscles from septic rats (16 hours after CLP) were treated with different concentrations of curcumin for 2 hours followed by measurement of tyrosine release. Results are means ±SEM with in each group except for “0 curcumin” which was pooled from 3 paired experiments (0 versus 50, 0 versus 100, and 0 versus 500 M). Results are expressed as % of control.
