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Mediators of Inflammation
Volume 2008, Article ID 614345, 7 pages
http://dx.doi.org/10.1155/2008/614345
Research Article

The NALP3/Cryopyrin-Inflammasome Complex is Expressed in LPS-Induced Ocular Inflammation

1Microbiology Department, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City 11340, Mexico
2Immunology Department, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City 11340, Mexico
3Pathology Department, Instituto Nacional de Pediatría, Mexico City 14410, Mexico

Received 16 April 2008; Accepted 1 July 2008

Academic Editor: Yvette Mándi

Copyright © 2008 José F. González-Benítez et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In the inflammosome complex, NALP3 or NALP1 binds to ASC and activates caspase-1 which induces IL-1 . In murine LPS-induced ocular inflammation, the production of IL-1 is increased. We suggest that NALP3- or NALP1-inflammasome complex can be participating in the LPS-induced ocular inflammation. In this work, eye, brain, testis, heart, spleen, and lung were obtained from C3H/HeN mice treated with LPS for 3 to 48 hours, and the expression of NALP1b, NALP3, ASC, caspase-1, IL-1 , and IL-18 was determined. Infiltrated leukocytes producing IL-1 in the anterior chamber were found at 12-hour posttreatment. A high upregulated expression of NALP3, ASC, caspase-1, IL-1 , and IL-18 was found at the same time when infiltrated leukocytes were observed. NALP1b was not detected in the eye of treated mice. NALP3 was also overexpressed in heart and lung. These results suggest that NALP3-, but not NALP1-inflammosome complex, is participating in the murine LPS-induced ocular inflammation.