The NALP3/Cryopyrin-Inflammasome Complex is Expressed in LPS-Induced Ocular Inflammation

Table 1

Sequence of oligonucleotides.

Gene	Sequence	Size amplified (pb)
β-Actin	Fw: 5^′-TGGAATCCTGTGGCATCCATGAAAC-3^′	324
	Rv: 5^′-TAAAACGCAGCTCAGTAACAGTCCG-3^′
NALP3	Fw: 5^′-CTGTGTGTGGGACTGAAGCAC-3^′	544
	Rv: 5^′-GCAGCCCTGCTGTTTCAGCAC-3^′
NALP1b	Fw: 5^′-TGGGATGGTTCTAGAAACGCC-3^′	602
	Rv: 5^′-AGGGTCCACTGATGTCACTCG-3^′
NALP5	Fw: 5^′-CCTGAGGAATCCAGAATGTGC-3^′	238
	Rv: 5^′-GCTTCCACAGGCCAATTATCC-3^′
NALP12	Fw: 5^′-GTACCAACTCCAACCTGATCG-3^′	512
	Rv: 5^′-GAAGTAGAGGCCAGATCTTTGC-3^′
ASC	Fw: 5^′-AGACATGGGCTTACAGGA-3^′	257
	Rv: 5^′-CTCCCTCATCTTGTCTTGG-3^′
Caspase-1	Fw: 5^′-TGAAAGAGGTGAAAGAATT-3^′	424
	Rv: 5^′-TCTCCAAGACACATTATCT-3^′
IL-1β	Fw: 5^′-TGGGCCTCAAAGGAAAGA-3^′	157
	Rv: 5^′-GGTGCTGATGTACCAGTT-3^′
IL-18	Fw: 5^′-CTGTACAACCGCAGTAATACGG-3^′	264
	Rv: 5^′-ACTCCATCTTGTTGTGTCCTGG-3^′
IL-6	Fw: 5^′-TCTCTGGGAAATCGTGGAAAT-3^′	308
	Rv: 5^′-TGTATCTCTCTGAAGGACTCTG-3^′
TNFα	Fw: 5^′-CCTCCCTCTCATCAGTTCTATGG-3^′	238
	Rv: 5^′-TGTCCCTTGAAGAGAACCTGG-3^′