Upregulation of Salmonella-Induced IL-6 Production in Caco-2 Cells by PJ-34, PARP-1 Inhibitor: Involvement of PI3K, p38 MAPK, ERK, JNK, and NF-B
Figure 1
(a) Effect of PJ-34 on Salmonella-induced IL-6 protein secretion. Caco-2 cells were left untreated (control), or treated with 5, 10, 20, 40, or 80 M PJ-34 (5, 10, 20, 40, 80PJ), or with a volume of DMSO (D) equivalent to the highest concentration of PJ-34. They were then uninfected (control and PJ only) or infected with the wild-type S. typhimurium strain SL1344 for 1 hour. Supernatant was analyzed by ELISA 6 hours later for IL-6. The amount of IL-6 produced is shown as the fold increase over uninfected, control cells. The results are representative of the results of two similar experiments. The data are the means standard deviations for three determinations. *. (b) Effect of PJ-34 on Salmonella-induced IL-6 mRNA. Caco-2 cells were left untreated, or treated with 5, 10, 20, 40, or 80 M PJ-34. They were then uninfected or infected with the wild-type S. Typhimurium strain SL1344 for 1 hour. Total RNA was prepared 3 hours later and analyzed by real-time quantitative PCR to estimate amounts of IL-6 transcript. The amount of IL-6 mRNA produced, normalized to the corresponding amount of GAPDH transcript, is shown as the fold increase over uninfected, control cells. The results are representative of the results of two similar experiments. The data are the means standard deviations for three determinations. *.
