Perforin Expression in Peripheral Blood Lymphatic Cells of Patients Subjected to Laparoscopic or Open Cholecystectomy
Figure 3
Percentage of total number of perforin positive cells (TPs) and double positive (perforin positive (P+) and surface marker positive) T lymphocytes (a) or NK cells (b) (mean percentage ± S.E.M.) in peripheral blood from patients subjected to classic or laparoscopic cholecystectomy before (two-colored columns) and after operation (one-colored column) compared to healthy control (first, blue column). O-statistical significance toward healthy control; X-statistical significance between the preoperative values in classic and laparoscopic cholecystectomy; *statistical significance between the preoperative and postoperative values in classic or laparoscopic cholecystectomy. One mark , two marks , three marks . (c) Representative flow cytometric profile of perforin expression on the gated CD8+ PBMNLC before (shadow area) and 24 hours after (solid line) laparoscopic cholecystectomy. Isotype control is shown as dotted line.
