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Mediators of Inflammation
Volume 2009 (2009), Article ID 183760, 5 pages
http://dx.doi.org/10.1155/2009/183760
Research Article

Regulation of Chemokine Production via Oxidative Pathway in HeLa Cells

Department of Clinical Neuroscience Oral and Maxillofacial Functional Rehabilitation, University of Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, Japan

Received 23 June 2009; Accepted 3 November 2009

Academic Editor: Hidde Bult

Copyright © 2009 Shinichiro Kina et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Inflammation is associated with disease progression and, by largely unknown mechanisms, has been said to drive oncogenesis. At inflamed sites, neutrophils deploy a potent antimicrobial arsenal that includes proteinases, antimicrobial peptides, and ROS. Reactive oxygen species (ROSs) induce chemokines. In the present study, the concentrations of IL-8 in culture supernatants of HeLa cells treated with ROS were determined by enzyme-linked immunosorbent assay. We used 𝑜 -phenanthroline to deplete F e 2 + in order to investigate the mechanisms through which ROSs induce IL-8 secretion in our system. The iron chelator 𝑜 -phenanthroline effectively inhibited H 2 O 2 -induced ERK2 activation. Enzyme-linked immunosorbent assays showed that IL-8 protein secretion was elevated in ROS-treated HeLa cells. When F e 2 + was removed from these cells, IL-8 secretion was inhibited. Collectively, these results indicate that F e 2 + -mediated Erk pathway activation is an important signal transduction pathway in ROS-induced IL-8 secretion in epithelial cells.