Interleukin (IL)-4 Induces Leukocyte Infiltration In Vivo by an Indirect Mechanism
Table 1
Analysis of the different analytes detected in the cell free exudates harvested from IL-4-induced air pouch1.
Analyte
3 hours
6 hours
9 hours
12 hours
BLC
0.23
1.10*
0.86
nd
CD30L
0.47
nd
1.71
*
nd
Eotaxin
0.70
1.10*
1.29
*
nd
Eotaxin-2
0.70
0.85
1.36
*
0.72
Fas Ligand
0.23
0.55
0.43
nd
Fractalkine
0.16
0.55
1.00
nd
G-CSF
0.31
nd
1.20
*
nd
GM-CSF
0.93
1.10*
0.43
0.36
IFN
0.47
1.10*
0.64
nd
IL-1
nd
1.10*
0.96
0.72
IL-1β
nd
nd
0.86
nd
IL-2
nd
nd
0.86
nd
IL-3
nd
1.10*
1.71
*
nd
IL-6
nd
nd
1.71
*
nd
IL-9
nd
0.55
1.71
*
nd
IL-10
nd
nd
1.71
*
nd
IL-12 p40/p70
0.35
0.55
1.03*
nd
IL-12p70
0.23
nd
2.00
*
0.72
IL-13
0.93
1.10*
2.14
*
0.72
IL-17
0.47
0.55
0.86
nd
I-TAC
nd
1.10*
1.20
*
0.72
KC
nd
0.55
1.71
*
1.44
*
Leptin
nd
1.10*
1.50
*
1.44
*
LIX/CXCL5
0.93
1.65
*
0.95
0.72
Lymphotactin
nd
0.70
1.19*
0.72
MCP-1/CCL2
0.47
7.25
*
1.54
*
nd
M-CSF
nd
nd
1.29
0.72
MIG
nd
1.46
*
0.94
nd
MIP-1
0.47
0.37
1.71
*
1.44
*
MIP-1
0.58
1.17*
1.05*
1.15*
RANTES
0.78
0.55
1.16*
0.12
SDF-1
0.47
nd
0.86
0.36
TCA-3
0.70
0.55
0.86
0.72
TECK
nd
nd
0.86
nd
TIMP-1
0.23
0.78
1.04*
nd
TIMP-2
0.47
nd
1.29
*
nd
TNF-
nd
0.55
0.86
0.72
sTNFR I
0.45
1.46
*
0.97
1.08*
sTNFR II
nd
0.55
0.86
nd
1Air pouches were created and 250 ng/ml IL-4 or buffer was administered into the pouch for the indicated periods of time. Pooled exudates ( mice) were used to probe the membranes (Mouse inflammation antibody array I) no more than two weeks after the in vivo experiments. Results are calculated as ratios, as described in Materials and Methods. nd, not detectable; *, ratio above 1; numbers in bold characters, ratio 1.2.
