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Mediators of Inflammation
Volume 2009, Article ID 285812, 9 pages
http://dx.doi.org/10.1155/2009/285812
Research Article

Differential between Protein and mRNA Expression of CCR7 and SSTR5 Receptors in Crohn's Disease Patients

1Laboratoire d'Innovation Thérapeutique, UMR 7200, Faculté de Pharmacie, Université de Strasbourg, 74 Route du Rhin, B.P. 24, 67401 Illkirch Cedex, France
2Centre d'Imagerie, Institut de Génétique et de Biologie Moléculaire et Cellulaire, INSERM U596, CNRS UMR7104, Université Louis Pasteur, 1 Rue Laurent Fries, 67404 Illkirch Cedex, France
3IFR 146 ICORE, EA 3919 (Biologie Moléculaire et Cellulaire de la Signalisation), Université de Caen—Basse Normandie, Hôpital Côte de Nacre, UFR de Médecine, 14032 Caen Cedex, France
4Service d'Hépato-Gastro-Entérologie et Nutrition, Pôle Reins-Digestif-Nutrition, Centre Hospitalier Universitaire, Avenue de la Côte de Nacre, B.P. 95182, 14033 Caen Cedex 09, France

Received 17 August 2009; Accepted 8 November 2009

Academic Editor: Donna-Marie McCafferty

Copyright © 2009 Nathalie Taquet et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Crohn's disease (CD) is a multifactorial chronic inflammatory bowel disease of unknown cause. The aim of the present study was to explore if mRNA over-expression of SSTR5 and CCR7 found in CD patients could be correlated to respective protein expression. When compared to healthy donors, SSTR5 was over-expressed 417 71 times in CD peripheral blood mononuclear cells (PBMCs). Flow cytometry experiments showed no correlation between mRNA and protein expression for SSTR5 in PBMCs. In an attempt to find a reason of such a high mRNA expression, SSTR5 present on CD PBMCs were tested and found as biologically active as on healthy cells. In biopsies of CD intestinal tissue, SSTR5 was not over-expressed but CCR7, unchanged in PBMCs, was over-expressed by 10 3 times in the lamina propria. Confocal microscopy showed a good correlation of CCR7 mRNA and protein expression in CD intestinal biopsies. Our data emphasize flow and image cytometry as impossible to circumvent in complement to molecular biology so to avoid false interpretation on receptor expressions. Once confirmed by further large-scale studies, our preliminary results suggest a role for SSTR5 and CCR7 in CD pathogenesis.